TY - JOUR
T1 - Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon
AU - Aston, Emily J.
AU - Mayor, Pedro
AU - Bowman, Dwight D.
AU - Mohammed, Hussni O.
AU - Liotta, Janice L.
AU - Kwok, Oliver
AU - Dubey, J. P.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them. © 2013 .
AB - Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them. © 2013 .
KW - Brocket deer (Mazama americana, Mazama gouazoubira)
KW - Filter paper
KW - Lowland tapir (Tapirus terrestris)
KW - Modified agglutination test
KW - Peccary (Pecari tajacu, Tayassu pecari)
KW - Toxoplasma gondii
UR - https://ddd.uab.cat/record/185051
U2 - https://doi.org/10.1016/j.ijppaw.2013.12.001
DO - https://doi.org/10.1016/j.ijppaw.2013.12.001
M3 - Article
VL - 3
SP - 15
EP - 19
JO - International Journal for Parasitology: Parasites and Wildlife
JF - International Journal for Parasitology: Parasites and Wildlife
SN - 2213-2244
ER -