Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon

Emily J. Aston; Pedro Mayor; Dwight D. Bowman; Hussni O. Mohammed; Janice L. Liotta; Oliver Kwok; J. P. Dubey

doi:https://doi.org/10.1016/j.ijppaw.2013.12.001

Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon

Emily J. Aston, Pedro Mayor, Dwight D. Bowman, Hussni O. Mohammed, Janice L. Liotta, Oliver Kwok, J. P. Dubey

Department of Animal Health and Anatomy

Research output: Contribution to journal › Article › Research › peer-review

20 Citations (Scopus)

Abstract

Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them. © 2013 .

Original language	English
Pages (from-to)	15-19
Journal	International Journal for Parasitology: Parasites and Wildlife
Volume	3
DOIs	https://doi.org/10.1016/j.ijppaw.2013.12.001
Publication status	Published - 1 Jan 2014

Keywords

Brocket deer (Mazama americana, Mazama gouazoubira)
Filter paper
Lowland tapir (Tapirus terrestris)
Modified agglutination test
Peccary (Pecari tajacu, Tayassu pecari)
Toxoplasma gondii

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title = "Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon",

abstract = "Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavar{\'i}-Mir{\'i}n River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them. {\textcopyright} 2013 .",

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author = "Aston, {Emily J.} and Pedro Mayor and Bowman, {Dwight D.} and Mohammed, {Hussni O.} and Liotta, {Janice L.} and Oliver Kwok and Dubey, {J. P.}",

year = "2014",

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doi = "https://doi.org/10.1016/j.ijppaw.2013.12.001",

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publisher = "Elsevier BV",

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AU - Aston, Emily J.

AU - Mayor, Pedro

AU - Bowman, Dwight D.

AU - Mohammed, Hussni O.

AU - Liotta, Janice L.

AU - Kwok, Oliver

AU - Dubey, J. P.

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N2 - Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them. © 2013 .

AB - Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them. © 2013 .

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KW - Lowland tapir (Tapirus terrestris)

KW - Modified agglutination test

KW - Peccary (Pecari tajacu, Tayassu pecari)

KW - Toxoplasma gondii

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EP - 19

JO - International Journal for Parasitology: Parasites and Wildlife

JF - International Journal for Parasitology: Parasites and Wildlife

SN - 2213-2244

ER -

Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon

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Keywords

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