Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodes

M. Cortina, M. J. Esplandiu, S. Alegret, M. del Valle

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

The measurement of urea was accomplished with an interdigitated electrode by using ac impedance spectroscopy to follow capacitance changes, which were produced during enzyme-catalyzed dissolution of a polymer coating. The employed coating was the enteric polymer Eudragit S-100, on which urease enzyme was immobilized by carbodiimide coupling. Urea was determined in the 0.02-2 M range, proving that the combination of the biofunctionalized electrode and the impedance technique results in an elegant approach for urea biosensing. © 2006 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)84-89
JournalSens. actuators, B, Chem.
Volume118
Issue number1-2
DOIs
Publication statusPublished - 25 Oct 2006

Keywords

  • Enteric polymer
  • Impedance spectroscopy
  • Interdigitated electrode
  • Urea biosensor

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