Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodes

M. Cortina; M. J. Esplandiu; S. Alegret; M. del Valle

doi:10.1016/j.snb.2006.04.062

Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodes

M. Cortina, M. J. Esplandiu, S. Alegret, M. del Valle

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

The measurement of urea was accomplished with an interdigitated electrode by using ac impedance spectroscopy to follow capacitance changes, which were produced during enzyme-catalyzed dissolution of a polymer coating. The employed coating was the enteric polymer Eudragit S-100, on which urease enzyme was immobilized by carbodiimide coupling. Urea was determined in the 0.02-2 M range, proving that the combination of the biofunctionalized electrode and the impedance technique results in an elegant approach for urea biosensing. © 2006 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	84-89
Journal	Sens. actuators, B, Chem.
Volume	118
Issue number	1-2
DOIs	https://doi.org/10.1016/j.snb.2006.04.062
Publication status	Published - 25 Oct 2006

Keywords

Enteric polymer
Impedance spectroscopy
Interdigitated electrode
Urea biosensor

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title = "Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodes",

abstract = "The measurement of urea was accomplished with an interdigitated electrode by using ac impedance spectroscopy to follow capacitance changes, which were produced during enzyme-catalyzed dissolution of a polymer coating. The employed coating was the enteric polymer Eudragit S-100, on which urease enzyme was immobilized by carbodiimide coupling. Urea was determined in the 0.02-2 M range, proving that the combination of the biofunctionalized electrode and the impedance technique results in an elegant approach for urea biosensing. {\textcopyright} 2006 Elsevier B.V. All rights reserved.",

keywords = "Enteric polymer, Impedance spectroscopy, Interdigitated electrode, Urea biosensor",

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AU - Cortina, M.

AU - Esplandiu, M. J.

AU - Alegret, S.

AU - del Valle, M.

PY - 2006/10/25

Y1 - 2006/10/25

N2 - The measurement of urea was accomplished with an interdigitated electrode by using ac impedance spectroscopy to follow capacitance changes, which were produced during enzyme-catalyzed dissolution of a polymer coating. The employed coating was the enteric polymer Eudragit S-100, on which urease enzyme was immobilized by carbodiimide coupling. Urea was determined in the 0.02-2 M range, proving that the combination of the biofunctionalized electrode and the impedance technique results in an elegant approach for urea biosensing. © 2006 Elsevier B.V. All rights reserved.

AB - The measurement of urea was accomplished with an interdigitated electrode by using ac impedance spectroscopy to follow capacitance changes, which were produced during enzyme-catalyzed dissolution of a polymer coating. The employed coating was the enteric polymer Eudragit S-100, on which urease enzyme was immobilized by carbodiimide coupling. Urea was determined in the 0.02-2 M range, proving that the combination of the biofunctionalized electrode and the impedance technique results in an elegant approach for urea biosensing. © 2006 Elsevier B.V. All rights reserved.

KW - Enteric polymer

KW - Impedance spectroscopy

KW - Interdigitated electrode

KW - Urea biosensor

U2 - 10.1016/j.snb.2006.04.062

DO - 10.1016/j.snb.2006.04.062

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