The measurement of urea was accomplished with an interdigitated electrode by using ac impedance spectroscopy to follow capacitance changes, which were produced during enzyme-catalyzed dissolution of a polymer coating. The employed coating was the enteric polymer Eudragit S-100, on which urease enzyme was immobilized by carbodiimide coupling. Urea was determined in the 0.02-2 M range, proving that the combination of the biofunctionalized electrode and the impedance technique results in an elegant approach for urea biosensing. © 2006 Elsevier B.V. All rights reserved.
|Journal||Sens. actuators, B, Chem.|
|Publication status||Published - 25 Oct 2006|
- Enteric polymer
- Impedance spectroscopy
- Interdigitated electrode
- Urea biosensor