Urea Determination in Human Sera with an Ammonium Ion Selective Electrode Made with Solid Inner Electric Contact and Immobilised Urease

G. Palleschi; M. Mascini; E. Martinez-Fabregas; S. Alegret

doi:10.1080/00032718808055500

Urea Determination in Human Sera with an Ammonium Ion Selective Electrode Made with Solid Inner Electric Contact and Immobilised Urease

G. Palleschi, M. Mascini, E. Martinez-Fabregas, S. Alegret

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

44 Citations (Scopus)

Abstract

A PVC matrix nonactin based membrane electrode assembled with a solid carbon-epoxy inner electric contact has been successfully used in ammonium and urea analysis. Different buffers have been tested and sodium and potassium interferences have been evaluated in order to obtain the best calibration curve for determination of ammonium and urea in serum samples. Three immobilization techniques for the enzyme urease have been compared. Nylon net immobilization procedure was found to have the best analytical features when coupled with this electrode and it has been selected for urea determination in sera. Results were in good agreement with those obtained with the clinical standard spectrophotometric method. © 1988, Taylor & Francis Group, LLC. All rights reserved.

Original language	English
Pages (from-to)	1115-1129
Journal	Analytical Letters
Volume	21
Issue number	7
DOIs	https://doi.org/10.1080/00032718808055500
Publication status	Published - 1 Jan 1988

Keywords

AMMONIUM ELECTRODE
NONACTIN
SOLID GRAPHITE CONTACT
UREA ANALYSIS
UREASE IMMOBILISATION

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10.1080/00032718808055500

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title = "Urea Determination in Human Sera with an Ammonium Ion Selective Electrode Made with Solid Inner Electric Contact and Immobilised Urease",

abstract = "A PVC matrix nonactin based membrane electrode assembled with a solid carbon-epoxy inner electric contact has been successfully used in ammonium and urea analysis. Different buffers have been tested and sodium and potassium interferences have been evaluated in order to obtain the best calibration curve for determination of ammonium and urea in serum samples. Three immobilization techniques for the enzyme urease have been compared. Nylon net immobilization procedure was found to have the best analytical features when coupled with this electrode and it has been selected for urea determination in sera. Results were in good agreement with those obtained with the clinical standard spectrophotometric method. {\textcopyright} 1988, Taylor & Francis Group, LLC. All rights reserved.",

keywords = "AMMONIUM ELECTRODE, NONACTIN, SOLID GRAPHITE CONTACT, UREA ANALYSIS, UREASE IMMOBILISATION",

author = "G. Palleschi and M. Mascini and E. Martinez-Fabregas and S. Alegret",

year = "1988",

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doi = "10.1080/00032718808055500",

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T1 - Urea Determination in Human Sera with an Ammonium Ion Selective Electrode Made with Solid Inner Electric Contact and Immobilised Urease

AU - Palleschi, G.

AU - Mascini, M.

AU - Martinez-Fabregas, E.

AU - Alegret, S.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - A PVC matrix nonactin based membrane electrode assembled with a solid carbon-epoxy inner electric contact has been successfully used in ammonium and urea analysis. Different buffers have been tested and sodium and potassium interferences have been evaluated in order to obtain the best calibration curve for determination of ammonium and urea in serum samples. Three immobilization techniques for the enzyme urease have been compared. Nylon net immobilization procedure was found to have the best analytical features when coupled with this electrode and it has been selected for urea determination in sera. Results were in good agreement with those obtained with the clinical standard spectrophotometric method. © 1988, Taylor & Francis Group, LLC. All rights reserved.

AB - A PVC matrix nonactin based membrane electrode assembled with a solid carbon-epoxy inner electric contact has been successfully used in ammonium and urea analysis. Different buffers have been tested and sodium and potassium interferences have been evaluated in order to obtain the best calibration curve for determination of ammonium and urea in serum samples. Three immobilization techniques for the enzyme urease have been compared. Nylon net immobilization procedure was found to have the best analytical features when coupled with this electrode and it has been selected for urea determination in sera. Results were in good agreement with those obtained with the clinical standard spectrophotometric method. © 1988, Taylor & Francis Group, LLC. All rights reserved.

KW - AMMONIUM ELECTRODE

KW - NONACTIN

KW - SOLID GRAPHITE CONTACT

KW - UREA ANALYSIS

KW - UREASE IMMOBILISATION

U2 - 10.1080/00032718808055500

DO - 10.1080/00032718808055500

M3 - Article

SN - 0003-2719

VL - 21

SP - 1115

EP - 1129

JO - Analytical Letters

JF - Analytical Letters

IS - 7

ER -

Urea Determination in Human Sera with an Ammonium Ion Selective Electrode Made with Solid Inner Electric Contact and Immobilised Urease

Abstract

Keywords

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