Unveiling the multifaceted mechanisms of antibacterial activity of buforin II and frenatin 2.3S peptides from skin micro-organs of the orinoco lime treefrog (Sphaenorhynchus lacteus)

Carolina Muñoz-Camargo, Vivian A. Salazar, Laura Barrero-Guevara, Sandra Camargo, Angela Mosquera, Helena Groot, Ester Boix

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Amphibian skin is a rich source of natural compounds with diverse antimicrobial and immune defense properties. Our previous studies showed that the frog skin secretions obtained by skin micro-organs from various species of Colombian anurans have antimicrobial activities against bacteria and viruses. We purified for the first time two antimicrobial peptides from the skin micro-organs of the Orinoco lime treefrog (Sphaenorhynchus lacteus) that correspond to Buforin II (BF2) and Frenatin 2.3S (F2.3S). Here, we have synthesized the two peptides and tested them against Gram-negative and Gram-positive bacteria, observing an effective bactericidal activity at micromolar concentrations. Evaluation of BF2 and F2.3S membrane destabilization activity on bacterial cell cultures and synthetic lipid bilayers reveals a distinct membrane interaction mechanism. BF2 agglutinates E. coli cells and synthetic vesicles, whereas F2.3S shows a high depolarization and membrane destabilization activities. Interestingly, we found that F2.3S is able to internalize within bacterial cells and can bind nucleic acids, as previously reported for BF2. Moreover, bacterial exposure to both peptides alters the expression profile of genes related to stress and resistance response. Overall, these results show the multifaceted mechanism of action of both antimicrobial peptides that can provide alternative tools in the fight against bacterial resistance.
Original languageEnglish
Article number2170
Pages (from-to)1-21
JournalInternational Journal of Molecular Sciences
Volume19
Issue number8
DOIs
Publication statusPublished - 1 Aug 2018

Keywords

  • Antibacterial activity
  • Antimicrobial peptides
  • Bacterial agglutination
  • Buforin II
  • DNA binding
  • Frenatin 2.3S
  • Frog skin secretions
  • Membrane leakage
  • Membrane translocation

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