Understanding trophic interactions of Orius spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods

Priscila Gomez-Polo, Oscar Alomar, Cristina Castañé, Thaïs Aznar-Fernández, Jonathan G. Lundgren, Josep Piñol, Nuria Agustí

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

© 2016 Society of Chemical Industry. BACKGROUND: The aphid Nasonovia ribisnigri (Mosley) (Hemiptera: Aphididae) and the thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) are common pests in Mediterranean lettuce crops, where Orius spp. are common generalist predators. Predation by Orius spp. was studied in a lettuce plot by conventional polymerase chain reaction (PCR) and real-time PCR analyses using specific primers of both main pests. Also, high-throughput sequencing was used to have a wider approach of the diet of these predators in natural field conditions. RESULTS: Molecular analyses indicated a higher predation on N. ribisnigri in spring and on F. occidentalis in summer. Predation on alternative prey, like Collembola, was also found in both seasons. Real-time PCR was more sensitive than conventional PCR in showing the target trophic links, whereas high-throughput sequencing revealed predation on other natural enemies - intraguild predation (IGP), showing other trophic interactions of Orius majusculus within the studied ecosystem. CONCLUSIONS: This study gives important information about the trophic relationships present in Mediterranean lettuce crops in different periods of the year. The detected predation by Orius spp. on alternative prey, as well as on other natural enemies, should be further investigated to clarify whether it adds or detracts to the biological control of N. ribisnigri and F. occidentalis. © 2015 Society of Chemical Industry
Original languageEnglish
Pages (from-to)272-279
JournalPest Management Science
Volume72
Issue number2
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • Conventional PCR
  • Gut-content analysis
  • High-throughput sequencing
  • Orius
  • Real-time PCR
  • Trophic relationships

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