Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics

José Luis Corchero, Brigitte Gasser, David Resina, Wesley Smith, Ermenegilda Parrilli, Felícitas Vázquez, Ibane Abasolo, Maria Giuliani, Jussi Jäntti, Pau Ferrer, Markku Saloheimo, Diethard Mattanovich, Simó Schwartz, Maria Luisa Tutino, Antonio Villaverde

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79 Citations (Scopus)

Abstract

Both conventional and innovative biomedical approaches require cost-effective protein drugs with high therapeutic potency, improved bioavailability, biocompatibility, stability and pharmacokinetics. The growing longevity of the human population, the increasing incidence and prevalence of age-related diseases and the better comprehension of genetic-linked disorders prompt to develop natural and engineered drugs addressed to fulfill emerging therapeutic demands. Conventional microbial systems have been for long time exploited to produce biotherapeutics, competing with animal cells due to easier operation and lower process costs. However, both biological platforms exhibit important drawbacks (mainly associated to intracellular retention of the product, lack of post-translational modifications and conformational stresses), that cannot be overcome through further strain optimization merely due to physiological constraints. The metabolic diversity among microorganisms offers a spectrum of unconventional hosts, that, being able to bypass some of these weaknesses, are under progressive incorporation into production pipelines. In this review we describe the main biological traits and potentials of emerging bacterial, yeast, fungal and microalgae systems, by comparing selected leading species with well established conventional organisms with a long run in protein drug production. © 2012 Elsevier Inc.
Original languageEnglish
Pages (from-to)140-153
JournalBiotechnology Advances
Volume31
Issue number2
DOIs
Publication statusPublished - 1 Mar 2013

Keywords

  • Aggregation
  • Biotherapeutics
  • Microbial cells
  • Protein folding
  • Protein production
  • Recombinant protein

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    Corchero, J. L., Gasser, B., Resina, D., Smith, W., Parrilli, E., Vázquez, F., Abasolo, I., Giuliani, M., Jäntti, J., Ferrer, P., Saloheimo, M., Mattanovich, D., Schwartz, S., Tutino, M. L., & Villaverde, A. (2013). Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics. Biotechnology Advances, 31(2), 140-153. https://doi.org/10.1016/j.biotechadv.2012.09.001