TY - JOUR
T1 - Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
AU - Pazos-Perez, Nicolas
AU - Pazos, Elena
AU - Catala, Carme
AU - Mir-Simon, Bernat
AU - Gomez-De Pedro, Sara
AU - Sagales, Juan
AU - Villanueva, Carlos
AU - Vila, Jordi
AU - Soriano, Alex
AU - Garcia De Abajo, F. Javier
AU - Alvarez-Puebla, Ramon A.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (∼1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (∼1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints.
AB - Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (∼1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (∼1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints.
U2 - https://doi.org/10.1038/srep29014
DO - https://doi.org/10.1038/srep29014
M3 - Article
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 29014
ER -