The depletion of tylosin from edible pig tissues was studied following 5 days of intramuscular (i.m.) administration of 10 mg/kg of tylosin to 16 crossbreed pigs. Animals were slaughtered at intervals after treatment and samples of muscle, kidney, liver, skin + fat, and injection site were collected and analysed by high-performance liquid chromatography (HPLC). Seven days after the completion of treatment, the concentration of tylosin in kidney, skin + fat, and at the injection site was higher than the European Union maximal residue limit (MRL) of 10O μg/kg. Tylosin residues in all tissues were below the quantification limit (50 μg/kg) at 10 and 14 days post-treatment. © 2002 Elsevier Science Ltd. All rights reserved.