Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches

Mercè Izquierdo-Serra, Marta Gascón-Moya, Jan J. Hirtz, Silvia Pittolo, Kira E. Poskanzer, Èric Ferrer, Ramon Alibés, Félix Busqué, Rafael Yuste, Jordi Hernando, Pau Gorostiza

Research output: Contribution to journalArticleResearchpeer-review

85 Citations (Scopus)


Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution. © 2014 American Chemical Society.
Original languageEnglish
Pages (from-to)8693-8701
JournalJournal of the American Chemical Society
Issue number24
Publication statusPublished - 18 Jun 2014


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