Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches

Mercè Izquierdo-Serra; Marta Gascón-Moya; Jan J. Hirtz; Silvia Pittolo; Kira E. Poskanzer; Èric Ferrer; Ramon Alibés; Félix Busqué; Rafael Yuste; Jordi Hernando; Pau Gorostiza

doi:10.1021/ja5026326

Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches

Mercè Izquierdo-Serra, Marta Gascón-Moya, Jan J. Hirtz, Silvia Pittolo, Kira E. Poskanzer, Èric Ferrer, Ramon Alibés, Félix Busqué, Rafael Yuste, Jordi Hernando, Pau Gorostiza

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

95 Citations (Scopus)

Abstract

Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution. © 2014 American Chemical Society.

Original language	English
Pages (from-to)	8693-8701
Journal	Journal of the American Chemical Society
Volume	136
Issue number	24
DOIs	https://doi.org/10.1021/ja5026326
Publication status	Published - 18 Jun 2014

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10.1021/ja5026326

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title = "Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches",

abstract = "Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution. {\textcopyright} 2014 American Chemical Society.",

author = "Merc{\`e} Izquierdo-Serra and Marta Gasc{\'o}n-Moya and Hirtz, {Jan J.} and Silvia Pittolo and Poskanzer, {Kira E.} and {\`E}ric Ferrer and Ramon Alib{\'e}s and F{\'e}lix Busqu{\'e} and Rafael Yuste and Jordi Hernando and Pau Gorostiza",

year = "2014",

month = jun,

day = "18",

doi = "10.1021/ja5026326",

language = "English",

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TY - JOUR

T1 - Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches

AU - Izquierdo-Serra, Mercè

AU - Gascón-Moya, Marta

AU - Hirtz, Jan J.

AU - Pittolo, Silvia

AU - Poskanzer, Kira E.

AU - Ferrer, Èric

AU - Alibés, Ramon

AU - Busqué, Félix

AU - Yuste, Rafael

AU - Hernando, Jordi

AU - Gorostiza, Pau

PY - 2014/6/18

Y1 - 2014/6/18

N2 - Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution. © 2014 American Chemical Society.

AB - Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution. © 2014 American Chemical Society.

U2 - 10.1021/ja5026326

DO - 10.1021/ja5026326

M3 - Article

SN - 0002-7863

VL - 136

SP - 8693

EP - 8701

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 24

ER -

Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches

Abstract

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