Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices

Elisabet Sarri; Joan Marc Servitja; Fernando Picatoste; Enrique Claro

doi:https://doi.org/10.1016/0014-5793(96)00906-4

Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices

Elisabet Sarri, Joan Marc Servitja, Fernando Picatoste, Enrique Claro

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

Noradrenaline- and ionomycin-stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [32P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using 3H-fatty acids showed that two different classes of phosphatidylcholine, separated by two-dimensional TLC, one enriched with high incorporation of [3H]arachidonic acid (B) and the other with [3H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual 32P-phospholipids extracted from [32P]Pi-labeled hippocampal slices mere incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [32P]phosphatidylcholine, i.e. A and B, produced a different band of [32P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.

Original language	English
Pages (from-to)	303-306
Journal	FEBS Letters
Volume	393
DOIs	https://doi.org/10.1016/0014-5793(96)00906-4
Publication status	Published - 16 Sep 1996

Keywords

Phosphatidylethanol
Phospholipase D
Rat hippocampus

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@article{cea58123a2fb4d69a184284fdbf9fbe9,

title = "Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices",

abstract = "Noradrenaline- and ionomycin-stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [32P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using 3H-fatty acids showed that two different classes of phosphatidylcholine, separated by two-dimensional TLC, one enriched with high incorporation of [3H]arachidonic acid (B) and the other with [3H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual 32P-phospholipids extracted from [32P]Pi-labeled hippocampal slices mere incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [32P]phosphatidylcholine, i.e. A and B, produced a different band of [32P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.",

keywords = "Phosphatidylethanol, Phospholipase D, Rat hippocampus",

author = "Elisabet Sarri and Servitja, {Joan Marc} and Fernando Picatoste and Enrique Claro",

year = "1996",

month = sep,

day = "16",

doi = "https://doi.org/10.1016/0014-5793(96)00906-4",

language = "English",

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pages = "303--306",

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TY - JOUR

T1 - Two phosphatidylethanol classes separated by thin layer chromatography are produced by phospholipase D in rat brain hippocampal slices

AU - Sarri, Elisabet

AU - Servitja, Joan Marc

AU - Picatoste, Fernando

AU - Claro, Enrique

PY - 1996/9/16

Y1 - 1996/9/16

N2 - Noradrenaline- and ionomycin-stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [32P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using 3H-fatty acids showed that two different classes of phosphatidylcholine, separated by two-dimensional TLC, one enriched with high incorporation of [3H]arachidonic acid (B) and the other with [3H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual 32P-phospholipids extracted from [32P]Pi-labeled hippocampal slices mere incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [32P]phosphatidylcholine, i.e. A and B, produced a different band of [32P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.

AB - Noradrenaline- and ionomycin-stimulated as well as basal phospholipase D activity from rat hippocampus produced, in the presence of ethanol, two different classes of [32P]phosphatidylethanol (designated I and II), which were separated by thin layer chromatography. Endogenous labeling experiments using 3H-fatty acids showed that two different classes of phosphatidylcholine, separated by two-dimensional TLC, one enriched with high incorporation of [3H]arachidonic acid (B) and the other with [3H]myristic acid (A), were the most likely sources for the two classes of phosphatidylethanol. Experiments where individual 32P-phospholipids extracted from [32P]Pi-labeled hippocampal slices mere incubated with cabbage phospholipase D, in the presence of ethanol, showed that each class of [32P]phosphatidylcholine, i.e. A and B, produced a different band of [32P]phosphatidylethanol, with the same mobility in TLC as phosphatidylethanol II and I, respectively.

KW - Phosphatidylethanol

KW - Phospholipase D

KW - Rat hippocampus

U2 - https://doi.org/10.1016/0014-5793(96)00906-4

DO - https://doi.org/10.1016/0014-5793(96)00906-4

M3 - Article

VL - 393

SP - 303

EP - 306

ER -