TTC7B emerges as a novel risk factor for ischemic stroke through the convergence of several genome-wide approaches

Tiago Krug, João Paulo Gabriel, Ricardo Taipa, Benedita V. Fonseca, Sophie Domingues-Montanari, Israel Fernandez-Cadenas, Helena Manso, Liliana O. Gouveia, João Sobral, Isabel Albergaria, Gisela Gaspar, Jordi Jiménez-Conde, Raquel Rabionet, José M. Ferro, Joan Montaner, Astrid M. Vicente, Mário Rui Silva, Ilda Matos, Gabriela Lopes, Sofia A. Oliveira

    Research output: Contribution to journalArticleResearchpeer-review

    43 Citations (Scopus)

    Abstract

    We hereby propose a novel approach to the identification of ischemic stroke (IS) susceptibility genes that involves converging data from several unbiased genetic and genomic tools. We tested the association between IS and genes differentially expressed between cases and controls, then determined which data mapped to previously reported linkage peaks and were nominally associated with stroke in published genome-wide association studies. We first performed gene expression profiling in peripheral blood mononuclear cells of 20 IS cases and 20 controls. Sixteen differentially expressed genes mapped to reported whole-genome linkage peaks, including the TTC7B gene, which has been associated with major cardiovascular disease. At the TTC7B locus, 46 tagging polymorphisms were tested for association in 565 Portuguese IS cases and 520 controls. Markers nominally associated in at least one test and defining associated haplotypes were then examined in 570 IS Spanish cases and 390 controls. Several polymorphisms and haplotypes in the intron 5-intron 6 region of TTC7B were also associated with IS risk in the Spanish and combined data sets. Multiple independent lines of evidence therefore support the role of TTC7B in stroke susceptibility, but further work is warranted to identify the exact risk variant and its pathogenic potential. © 2012 ISCBFM All rights reserved.
    Original languageEnglish
    Pages (from-to)1061-1072
    JournalJournal of Cerebral Blood Flow and Metabolism
    Volume32
    Issue number6
    DOIs
    Publication statusPublished - 1 Jun 2012

    Keywords

    • cerebrovascular disease
    • gene expression profiling
    • genomic convergence
    • PBMCs
    • susceptibility genes

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