The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets, leucocytes, transforming growth factor beta-3 (TGF-β3) and nitric oxide (NO). Whole blood samples were collected from clinically healthy horses and processed using single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C and PC-D, which were analyzed using a flow cytometry hematology system for haemogram. Concentrations of TGF-β3 were determined in all the samples by ELISA and NO levels were determined by Greiss reaction. Platelet and leukocyte concentrations for each PC and whole blood were statistically different (P < 0.05). Levels of TGF-β3 and NO for each PC and plasma were similar. In conclusion, the single and double centrifugation tube methods are reliable for concentrating equine platelets. However, TGF-β3 levels do not depend of the number of platelets or leucocytes concentrated with both centrifugation tube procedures.
|Journal||Archivos de Medicina Veterinaria|
|Publication status||Published - 10 Oct 2008|
- Nitric oxide
- Transforming growth factor beta-3