Transforming growth factor beta-3 and nitric oxide levels in four autologous platelet concentrates and plasma derived from equine blood

J. U. Carmona, D. Argüelles, M. Prades

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13 Citations (Scopus)

Abstract

The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets, leucocytes, transforming growth factor beta-3 (TGF-β3) and nitric oxide (NO). Whole blood samples were collected from clinically healthy horses and processed using single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C and PC-D, which were analyzed using a flow cytometry hematology system for haemogram. Concentrations of TGF-β3 were determined in all the samples by ELISA and NO levels were determined by Greiss reaction. Platelet and leukocyte concentrations for each PC and whole blood were statistically different (P < 0.05). Levels of TGF-β3 and NO for each PC and plasma were similar. In conclusion, the single and double centrifugation tube methods are reliable for concentrating equine platelets. However, TGF-β3 levels do not depend of the number of platelets or leucocytes concentrated with both centrifugation tube procedures.
Original languageEnglish
Pages (from-to)155-160
JournalArchivos de Medicina Veterinaria
Volume40
Issue number2
Publication statusPublished - 10 Oct 2008

Keywords

  • Equine
  • Nitric oxide
  • Platelet
  • Transforming growth factor beta-3

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