Trans-infection but not infection from within endosomal compartments after cell-to-cell HIV-1 transfer to CD4, T cells

Marc Permanyer, Ester Ballana, Roger Badia, Eduardo Pauls, Bonaventura Clotet, José A. Esté

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Cellular contacts between HIV-1-infected donor cells and uninfected primary CD4- T lymphocytes lead to virus transfer into endosomes. Recent evidence suggests that HIV particles may fuse with endosomal membranes to initiate a productive infection. To explore the role of endocytosis in the entry and replication of HIV, we evaluated the infectivity of transferred HIV particles in a cell-to-cell culture model of virus transmission. Endocytosed virus led to productive infection of cells, except when cells were cultured in the presence of the antigp120 mAb IgGb12, an agent that blocks virus attachment to CD4, suggesting that endocytosed virus was recycled to the outer cell surface. Confocal microscopy confirmed the colocalization of internalized virus antigen and the endosomal marker dynamin. Additionally, virus transfer, fusion, or productive infection was not blocked by dynasore, dynamin-dependent endosome-scission inhibitor, at subtoxic concentrations, suggesting that the early capture of virus into intracellular compartments did not depend on endosomal maturation. Our results suggest that endocytosis is not a mechanism of infection of primary CD4 T cells, but may serve as a reservoir capable of inducing trans-infection of cells after the release of HIV particles to the extracellular environment. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
Original languageEnglish
Pages (from-to)32017-32026
JournalJournal of Biological Chemistry
Volume287
Issue number38
DOIs
Publication statusPublished - 14 Sep 2012

Fingerprint Dive into the research topics of 'Trans-infection but not infection from within endosomal compartments after cell-to-cell HIV-1 transfer to CD<sup>4</sup>, T cells'. Together they form a unique fingerprint.

Cite this