TY - JOUR
T1 - Traceability of human sperm samples by direct tagging with polysilicon microbarcodes
AU - Novo, Sergi
AU - Mora-Espí, Inma
AU - Gómez-Martínez, Rodrigo
AU - Barrios, Leonardo
AU - Ibáñez, Elena
AU - Such, Xavier
AU - Duch, Marta
AU - Mora, Xavier
AU - Plaza, José Antonio
AU - Nogués, Carme
PY - 2015/8/1
Y1 - 2015/8/1
N2 - © 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. The increasing number of patients undergoing assisted reproductive technology (ART) treatments and of cycles performed in fertility centres has led to some traceability errors. Although the incidence of mismatching errors is extremely low, any error is unacceptable, therefore different strategies have been developed to further minimize these errors, such as manual double-witnessing or electronic witnessing systems. More recently, our group developed a direct tagging method consisting of attaching microbarcodes directly to the zona pellucida of human oocytes/embryos. Here, this method is taken a step further by using these microbarcodes to tag human semen samples, demonstrating that the barcodes are not toxic and do not interfere in the selection of motile spermatozoa nor in the cryopreservation of the sperm samples. In addition, when this tagging system was applied to an animal model (rabbit), pregnancy rate and kitten viability were not affected.
AB - © 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. The increasing number of patients undergoing assisted reproductive technology (ART) treatments and of cycles performed in fertility centres has led to some traceability errors. Although the incidence of mismatching errors is extremely low, any error is unacceptable, therefore different strategies have been developed to further minimize these errors, such as manual double-witnessing or electronic witnessing systems. More recently, our group developed a direct tagging method consisting of attaching microbarcodes directly to the zona pellucida of human oocytes/embryos. Here, this method is taken a step further by using these microbarcodes to tag human semen samples, demonstrating that the barcodes are not toxic and do not interfere in the selection of motile spermatozoa nor in the cryopreservation of the sperm samples. In addition, when this tagging system was applied to an animal model (rabbit), pregnancy rate and kitten viability were not affected.
KW - assisted reproduction
KW - barcodes
KW - spermatozoa
KW - traceability
U2 - 10.1016/j.rbmo.2015.04.012
DO - 10.1016/j.rbmo.2015.04.012
M3 - Article
SN - 1472-6483
VL - 31
SP - 162
EP - 170
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
IS - 2
ER -
