Towards understanding the Regulation of Histone H1 Somatic Subtypes with OMICs

Inma Ponte, Marta Andrés, Albert Jordan, Alicia Roque*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review


Histone H1 is involved in the regulation of chromatin higher-order structure and compaction. In humans, histone H1 is a multigene family with seven subtypes differentially expressed in somatic cells. Which are the regulatory mechanisms that determine the variability of the H1 complement is a long-standing biological question regarding histone H1. We have used a new approach based on the integration of OMICs data to address this issue. We have examined the 3D-chromatin structure, the binding of transcription factors (TFs), and the expression of somatic H1 genes in human cell lines, using data from public repositories, such as ENCODE. Analysis of Hi-C, ChIP-seq, and RNA-seq data, have revealed that transcriptional control has a greater impact on H1 regulation than previously thought. Somatic H1 genes located in topologically associated domains (TADS) show higher expression than in boundary regions. H1 genes are targeted by a variable number of transcription factors including cell cycle-related TFs, and tissue-specific TFs, suggesting a fine-tuned, subtype-specific transcriptional control. We describe, for the first time, that all H1 somatic subtypes are under transcriptional co-regulation. The replication-independent subtypes, which are encoded in different chromosomes isolated from other histone genes, are also co-regulated with the rest of the somatic H1 genes, indicating that transcriptional co-regulation extends beyond the histone cluster. Transcriptional control and transcriptional co-regulation explain, at least in part, the variability of H1 complement, the fluctuations of H1 subtypes during development, and also the compensatory effects observed, in model systems, after perturbation of one or more H1 subtypes. (C) 2020 Elsevier Ltd. All rights reserved.

Original languageEnglish
Article number166734
Pages (from-to)166734
Number of pages18
JournalJournal of Molecular Biology
Issue number2
Publication statusPublished - 1 Jan 2021


  • chromatin compartments
  • co-regulation
  • subtype functional differentiation
  • topologically associated domains (TADs)
  • transcription factors
  • Cell Line
  • Promoter Regions, Genetic
  • Humans
  • Chromosome Mapping
  • Gene Expression Profiling
  • Genomics/methods
  • Histones/chemistry
  • Transcription Factors/metabolism
  • Chromatin/genetics
  • Protein Binding
  • Proteomics/methods


Dive into the research topics of 'Towards understanding the Regulation of Histone H1 Somatic Subtypes with OMICs'. Together they form a unique fingerprint.

Cite this