Tolerance of Escherichia coli β-galactosidase C-terminus to different- sized fusions

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Abstract

The tolerance of the β-galactosidase C-terminus to foreign protein fusions has been explored by using different-sized derivatives of the chimeric protein LACVP1. While the molecular mass of the partner domain shows a minor influence on protein toxicity for the producing E. coli cells, it dramatically affects the proteolytic susceptibility of the whole fusion. Surprisingly, the observed structural modulation of proteolysis is not an all-or-nothing process, but it exhibits a continuous effect concomitantly with the length of the fusion. The conformational effects caused by increasingly sized partners seem to progressively expose cryptic protease target sites, initiating a proteolytic cascade that dramatically reduces the yield of the recombinant protein.
Original languageEnglish
Pages (from-to)644-649
JournalBiotechnology and Bioengineering
Volume64
Issue number6
DOIs
Publication statusPublished - 20 Sep 1999

Keywords

  • β-galactosidase
  • Escherichia coli
  • Fusion proteins
  • Misfolding
  • Proteolysis
  • Toxicity

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