The process of β-cell destruction in IDDM is mediated, in part, by CD8+ T-cells. Structural characterization of HLA-I-bound self-peptides presented by the human β- cell line HP-62 was performed to identify possible tissue-specific autoantigens in the context of CD8+ T- cell/HLA-I interactions. The sequences of the β-cell line HLA-I-bound peptides were compared with sequence databases. Six of the obtained sequences showed homology to known precursor proteins, three of which-GLUT2 receptor, phosphatidylinositol-glycan-specific phospholipase D, and 5- hydroxytryptamine-1F receptor-have a limited, tissue-specific expression. These HLA-bound self-peptides may be part of a pool of autoantigens recognized by β-cell reactive cytotoxic T-cells.
|Publication status||Published - 9 Dec 1996|