Tissue macroarrays ("microchops") for gene expression analysis

PL Fernandez, Iracema Nayach, E Fernandez, L Fresno, A Palacin, M. Farre, E Campo, A Cardesa

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15 Citations (Scopus)

Abstract

We describe a simple system of tissue arraying with multiple tissue fragments obtained with a biopsy punch from selected areas of paraffin blocks. The new blocks thus constructed allow multiple tissue sections in which the uniform shape of the fragments coupled with a geometrical display and a significant amount of tissue per case allows a dependable, cost-effective way to screen tumors or other kinds of tissues with techniques such as immunohistochemistry. This system avoids the disadvantages of previous laborious methods of tissue arraying, such as expensive equipment and scarce tissue sampling, and it can be implemented in any institution with minimal cost and elaboration.

Original languageEnglish
Pages (from-to)591-594
Number of pages4
JournalVirchows Arch.
Volume438
Issue number6
DOIs
Publication statusPublished - Jun 2001

Keywords

  • tissue array
  • immunohistochemistry
  • microchops
  • MICROARRAYS
  • SPECIMENS
  • CANCER
  • BLOCK

Cite this

Fernandez, PL., Nayach, I., Fernandez, E., Fresno, L., Palacin, A., Farre, M., Campo, E., & Cardesa, A. (2001). Tissue macroarrays ("microchops") for gene expression analysis. Virchows Arch., 438(6), 591-594. https://doi.org/10.1007/s004280100393