Time-sharing evolution and sensitivity enhancements in 2D HSQC-TOCSY and HSQMBC experiments

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Modifications of time-shared (TS) HSQC-like experiments originally developed by Griesinger and co-workers (Sattler M, Maurer M, Schleucher J, Griesinger C. J. Biomol. NMR 1995; 5: 97) are proposed to extract different types of information from a single NMR pulse scheme. It is shown that simultaneous acquisition of 1H,13C and 1H, 15N HSQC-TOCSY and HSQMBC experiments can afford experimental sensitivity enhancements of 20-40% with respect to the separate acquisition of individual 13C or 15N data. In addition, the incorporation of a number of independent editing elements can be easily used for different purposes, for instance, to assign unambiguously 1H, 13C, and 15N chemical shifts, to differentiate directly from relayed cross-peaks, or to measure simultaneously long-range proton-carbon and proton-nitrogen coupling constants. The suggested methodologies can be applied to many different classes of nitrogen-containing compounds and illustrative examples are provided for the peptide cyclosporine as a demonstration of the performance of such experiments. Copyright © 2006 John Wiley & Sons, Ltd.
Original languageEnglish
Pages (from-to)1031-1036
JournalMagnetic Resonance in Chemistry
Issue number11
Publication statusPublished - 1 Nov 2006


  • Heteronuclear coupling constants
  • IPAP
  • NMR spectroscopy
  • Sensitivity enhancement
  • Time-sharing


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