Abstract
The aim of the present work is to compare various thick-film configurations as well as the different enzyme immobilisation techniques used in the design of a pesticide biosensor. Three different strategies are employed for enzyme immobilisation. According to the first strategy the enzyme layer is applied manually onto the transducer (graphite-epoxy composite screen-printed onto copper tracks produced by photolithography technique) and then the immobilisation of enzyme via cross-linking with glutaraldehyde was performed. In the second strategy the biosensor fabrication is based on the screen-printing of an enzyme-hydroxyethyl cellulose (HEC) paste onto the same transducer. The third strategy consists in the biological material incorporation into the graphite-epoxy paste forming a biocomposite. The enzyme remains immobilised in the bulk of the paste which is screen-printed over copper tracks producing the biosensor in a single-step procedure. Analytical performances of biosensors designed by these three different configurations are compared. © 2001 Elsevier Science B.V. All rights reserved.
Original language | English |
---|---|
Pages (from-to) | 48-57 |
Journal | Sensors and Actuators, B: Chemical |
Volume | 79 |
Issue number | 1 |
DOIs | |
Publication status | Published - 25 Sep 2001 |
Keywords
- Biocomposite
- Cholinesterase
- Graphite-epoxy composite
- Pesticide
- Screen-printing
- Thick-film biosensors