Thick-film biosensors for pesticides produced by screen-printing of graphite-epoxy composite and biocomposite pastes

Miquel Albareda-Sirvent, Arben Merkoçi, Salvador Alegret

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

The aim of the present work is to compare various thick-film configurations as well as the different enzyme immobilisation techniques used in the design of a pesticide biosensor. Three different strategies are employed for enzyme immobilisation. According to the first strategy the enzyme layer is applied manually onto the transducer (graphite-epoxy composite screen-printed onto copper tracks produced by photolithography technique) and then the immobilisation of enzyme via cross-linking with glutaraldehyde was performed. In the second strategy the biosensor fabrication is based on the screen-printing of an enzyme-hydroxyethyl cellulose (HEC) paste onto the same transducer. The third strategy consists in the biological material incorporation into the graphite-epoxy paste forming a biocomposite. The enzyme remains immobilised in the bulk of the paste which is screen-printed over copper tracks producing the biosensor in a single-step procedure. Analytical performances of biosensors designed by these three different configurations are compared. © 2001 Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)48-57
JournalSensors and Actuators, B: Chemical
Volume79
Issue number1
DOIs
Publication statusPublished - 25 Sep 2001

Keywords

  • Biocomposite
  • Cholinesterase
  • Graphite-epoxy composite
  • Pesticide
  • Screen-printing
  • Thick-film biosensors

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