Theoretical study on two-step mechanisms of peptide release in the ribosome

Carles Acosta-Silva, Joan Bertran, Vicenç Branchadell, Antoni Oliva

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

A quantum mechanical study of different two-step mechanisms of peptide release in the ribosome has been carried out using the M06-2X density functional. Reoptimization with MP2 has also been carried out for the stationary points of some selected mechanisms. The uncatalyzed processes in solution have been treated with the SMD solvation model. From the results obtained in this paper for the peptide release process we can conclude that the energy barriers for the two-step mechanisms are lower than the ones for the concerted process, that the 2′OH plays also an important role in the catalytic process and that the side chain does not only accommodate a nucleophilic water molecule in the PTC, but it also contributes to activate this molecule through electron transfer to the water oxygen. We have also found that the second step is the rate-determining one, and that the two most favorable mechanisms, in which a water or a formamide molecule is added, follow a quite different strategy to catalyze the reaction. The main conclusion of our work is that the two-step mechanisms cannot be disregarded, since they can contribute to clarify the complex and yet unsolved problem of the mechanism of the peptide release process. © 2014 American Chemical Society.
Original languageEnglish
Pages (from-to)5717-5729
JournalJournal of Physical Chemistry B
Volume118
Issue number21
DOIs
Publication statusPublished - 29 May 2014

Fingerprint Dive into the research topics of 'Theoretical study on two-step mechanisms of peptide release in the ribosome'. Together they form a unique fingerprint.

Cite this