The wing-spot and the comet tests as useful assays detecting genotoxicity in Drosophila

Ricard Marcos; Erico R. Carmona

doi:https://doi.org/10.1007/978-1-62703-529-3_23

The wing-spot and the comet tests as useful assays detecting genotoxicity in Drosophila

Ricard Marcos, Erico R. Carmona

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16 Citations (Scopus)

Abstract

In spite of its pioneer use in detecting mutational processes, Drosophila has yet an important role in studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer and no assays exist to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila. © Springer Science+Business Media, New York 2013.

Original language	English
Pages (from-to)	417-427
Journal	Methods in Molecular Biology
Volume	1044
DOIs	https://doi.org/10.1007/978-1-62703-529-3_23
Publication status	Published - 1 Jan 2013

Keywords

Comet assay
DNA damage
Drosophila
Genotoxicity
Wing-spot test

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https://doi.org/10.1007/978-1-62703-529-3_23

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AB - In spite of its pioneer use in detecting mutational processes, Drosophila has yet an important role in studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer and no assays exist to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila. © Springer Science+Business Media, New York 2013.

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KW - DNA damage

KW - Drosophila

KW - Genotoxicity

KW - Wing-spot test

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The wing-spot and the comet tests as useful assays detecting genotoxicity in Drosophila

Abstract

Keywords

UN SDGs

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