The use of quantum dots for immunochemistry applications

Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, Carme Nogués

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Immunocytochemistry and histochemistry are two most valuable immunochemistry techniques routinely used in biological laboratories. These techniques rely on the use of antibodies to label epitopes of interest in cells. At present, there is a wide range of commercially available organic dyes for labeling antibodies. However, limited extinction coefficients of organic dyes often make it difficult to achieve the optimal exposure and therefore fluorescence detection limit. Quantum dots (QDs) are fluorescent semiconductor nanocrystals which are advantageous over the organic fluorescent dyes in many aspects, principally their long-term luminescence stability, high brightness, and multicolor detection. Here, we describe the use of QDs for immunocytochemistry applications. We used three different antibodies-anti- β-tubulin monoclonal antibody for visualizing the microtubule network, the GM130 antibody for staining the Golgi complex, and the EEA1 antibody for detecting the endosomal system. We use the anti-mouse IgG antibody directly conjugated to QD655 quantum dots or anti-mouse IgG conjugated to biotin for tertiary detection with streptavidin-conjugated QD655. © 2012 Springer Science+Business Media, LLC.
Original languageEnglish
Pages (from-to)185-192
JournalMethods in Molecular Biology
Volume906
DOIs
Publication statusPublished - 15 Aug 2012

Keywords

  • Antibody
  • Cell culture
  • Confocal laser scanning microscope
  • Fluorescence
  • Immunocytochemistry
  • Quantum dots

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