The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation

Lourdes Serrano, Paloma Martínez-Redondo, Anna Marazuela-Duque, Berta N. Vazquez, Scott J. Dooley, Philipp Voigt, David B. Beck, Noriko Kane-Goldsmith, Qiang Tong, Rosa M. Rabanal, Dolors Fondevila, Purificación Muñoz, Marcus Krüger, Jay A. Tischfield, Alejandro Vaquero

Research output: Contribution to journalArticleResearchpeer-review

144 Citations (Scopus)

Abstract

The establishment of the epigenetic mark H4K20me1 (monomethylation of H4K20) by PR-Set7 during G2/M directly impacts S-phase progression and genome stability. However, the mechanisms involved in the regulation of this event are not well understood. Here we show that SirT2 regulates H4K20me1 deposition through the deacetylation of H4K16Ac (acetylation of H4K16) and determines the levels of H4K20me2/3 throughout the cell cycle. SirT2 binds and deacetylates PR-Set7 at K90, modulating its chromatin localization. Consistently, SirT2 depletion significantly reduces PR-Set7 chromatin levels, alters the size and number of PR-Set7 foci, and decreases the overall mitotic deposition of H4K20me1. Upon stress, the interaction between SirT2 and PR-Set7 increases along with the H4K20me1 levels, suggesting a novel mitotic checkpoint mechanism. SirT2 loss in mice induces significant defects associated with defective H4K20me1-3 levels. Accordingly, SirT2-deficient animals exhibit genomic instability and chromosomal aberrations and are prone to tumorigenesis. Our studies suggest that the dynamic cross-talk between the environment and the genome during mitosis determines the fate of the subsequent cell cycle. © 2013 by Cold Spring Harbor Laboratory Press.
Original languageEnglish
Pages (from-to)639-653
JournalGenes and Development
Volume27
DOIs
Publication statusPublished - 1 Mar 2013

Keywords

  • Epigenetics
  • Genome instability
  • H4K20me1
  • PR-Set7
  • SIRT2
  • Sirtuins

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    Serrano, L., Martínez-Redondo, P., Marazuela-Duque, A., Vazquez, B. N., Dooley, S. J., Voigt, P., Beck, D. B., Kane-Goldsmith, N., Tong, Q., Rabanal, R. M., Fondevila, D., Muñoz, P., Krüger, M., Tischfield, J. A., & Vaquero, A. (2013). The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation. Genes and Development, 27, 639-653. https://doi.org/10.1101/gad.211342.112