The photoreactivity of 2,6-difluoro-4-nitroanisole in the presence of nucleophiles suggests that 2,6-difluoro-4-nitrophenyl ethers can constitute improved biochemical photoprobes for use in photoaffinity labeling reagents, specially for proteins where a number of good nucleophiles are present. Almost no photoreduction processes are observed, and one of the fluorine atoms is never replaced. This would allow the detection of the labeled fragments in a photoaffinity labeling experiment by simple NMR methods. © 1995.
Casado, F., Cervera, M., Marquet, J., & Moreno-Mañas, M. (1995). The Search for Biochemical Photoprobes. IV. The Photoreactions of 2,6-Difluoro-4-nitroanisole with Nucleophiles. Tetrahedron, 51(23), 6557-6564. https://doi.org/10.1016/0040-4020(95)00343-7