The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

J. Marquet; L. Rafecas; A. Cantos; M. Moreno; M. Cervera; F. Casado; M.V. Nogués; C.M. Cuchillo

doi:10.1016/S0040-4020(01)85819-1

The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

J. Marquet, L. Rafecas, A. Cantos, M. Moreno, M. Cervera, F. Casado, M.V. Nogués, C.M. Cuchillo

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Abstract

The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. © 1993.

Original language	English
Pages (from-to)	1297-1306
Journal	Tetrahedron
Volume	49
Issue number	6
DOIs	https://doi.org/10.1016/S0040-4020(01)85819-1
Publication status	Published - 5 Feb 1993

Keywords

Nitrophenyl Ethers
Photoprobes
Photosubstitution
Ribonuclease

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10.1016/S0040-4020(01)85819-1

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Marquet, J., Rafecas, L., Cantos, A., Moreno, M., Cervera, M., Casado, F., Nogués, M. V., & Cuchillo, C. M. (1993). The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles. Tetrahedron, 49(6), 1297-1306. https://doi.org/10.1016/S0040-4020(01)85819-1

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abstract = "The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. {\textcopyright} 1993.",

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author = "J. Marquet and L. Rafecas and A. Cantos and M. Moreno and M. Cervera and F. Casado and M.V. Nogu{\'e}s and C.M. Cuchillo",

year = "1993",

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doi = "10.1016/S0040-4020(01)85819-1",

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Marquet, J, Rafecas, L, Cantos, A, Moreno, M, Cervera, M, Casado, F, Nogués, MV & Cuchillo, CM 1993, 'The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.', Tetrahedron, vol. 49, no. 6, pp. 1297-1306. https://doi.org/10.1016/S0040-4020(01)85819-1

TY - JOUR

T1 - The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

AU - Marquet, J.

AU - Rafecas, L.

AU - Cantos, A.

AU - Moreno, M.

AU - Cervera, M.

AU - Casado, F.

AU - Nogués, M.V.

AU - Cuchillo, C.M.

PY - 1993/2/5

Y1 - 1993/2/5

N2 - The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. © 1993.

AB - The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. © 1993.

KW - Nitrophenyl Ethers

KW - Photoprobes

KW - Photosubstitution

KW - Ribonuclease

UR - https://www.scopus.com/pages/publications/0027529502

U2 - 10.1016/S0040-4020(01)85819-1

DO - 10.1016/S0040-4020(01)85819-1

M3 - Article

SN - 0040-4020

VL - 49

SP - 1297

EP - 1306

JO - Tetrahedron

JF - Tetrahedron

IS - 6

ER -

The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

Abstract

Keywords

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