The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

Jorge Marquet; Lourdes Rafecas; Albert Cantos; Marcial Moreno-Mañas; Maria Cervera; Francisco Casado; M. Victòria Nogués; Claudi M. Cuchillo

doi:10.1016/S0040-4020(01)85819-1

The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

Jorge Marquet, Lourdes Rafecas, Albert Cantos, Marcial Moreno-Mañas, Maria Cervera, Francisco Casado, M. Victòria Nogués, Claudi M. Cuchillo

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. © 1993.

Original language	English
Pages (from-to)	1297-1306
Journal	Tetrahedron
Volume	49
Issue number	6
DOIs	https://doi.org/10.1016/S0040-4020(01)85819-1
Publication status	Published - 5 Feb 1993

Keywords

Nitrophenyl Ethers
Photoprobes
Photosubstitution
Ribonuclease

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10.1016/S0040-4020(01)85819-1

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Marquet, J., Rafecas, L., Cantos, A., Moreno-Mañas, M., Cervera, M., Casado, F., Nogués, M. V., & Cuchillo, C. M. (1993). The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles. Tetrahedron, 49(6), 1297-1306. https://doi.org/10.1016/S0040-4020(01)85819-1

Marquet, Jorge ; Rafecas, Lourdes ; Cantos, Albert ; Moreno-Mañas, Marcial ; Cervera, Maria ; Casado, Francisco ; Nogués, M. Victòria ; Cuchillo, Claudi M. / The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles. In: Tetrahedron. 1993 ; Vol. 49, No. 6. pp. 1297-1306.

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abstract = "The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. {\textcopyright} 1993.",

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Marquet, J, Rafecas, L, Cantos, A, Moreno-Mañas, M, Cervera, M, Casado, F, Nogués, MV & Cuchillo, CM 1993, 'The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.', Tetrahedron, vol. 49, no. 6, pp. 1297-1306. https://doi.org/10.1016/S0040-4020(01)85819-1

The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles. / Marquet, Jorge; Rafecas, Lourdes; Cantos, Albert; Moreno-Mañas, Marcial; Cervera, Maria; Casado, Francisco; Nogués, M. Victòria; Cuchillo, Claudi M.

In: Tetrahedron, Vol. 49, No. 6, 05.02.1993, p. 1297-1306.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Marquet, Jorge

AU - Rafecas, Lourdes

AU - Cantos, Albert

AU - Moreno-Mañas, Marcial

AU - Cervera, Maria

AU - Casado, Francisco

AU - Nogués, M. Victòria

AU - Cuchillo, Claudi M.

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AB - The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. © 1993.

KW - Nitrophenyl Ethers

KW - Photoprobes

KW - Photosubstitution

KW - Ribonuclease

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DO - 10.1016/S0040-4020(01)85819-1

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JO - Tetrahedron

JF - Tetrahedron

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ER -

The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

Abstract

Keywords

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