The role of phytochelatins in constitutive and adaptive heavy metal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes

Henk Schat, Mercè Llugany, Riet Vooijs, Jeanette Hartley-Whitaker, Petra M. Bleeker

Research output: Contribution to journalArticleResearchpeer-review

283 Citations (Scopus)

Abstract

Using the γ-glutamylcysteine synthetase inhibitor, L-buthionine-[S,R]-sulphoximine (BSO), the role for phytochelatins (PCs) was evaluated in Cu, Cd, Zn, As, Ni, and Co tolerance in non-metallicolous and metallicolous, hypertolerant populations of Silene vulgaris (Moench) Garcke, Thlaspi caerulescens J.&C. Presl., Holcus lanatus L., and Agrostis castellana Boiss. et Reuter. Based on plant-internal PC-thiol to metal molar ratios, the metals' tendency to induce PC accumulation decreased in the order As/Cd/Cu > Zn > Ni/Co, and was consistently higher in non-metallicolous plants than in hypertolerant ones, except for the case of As. The sensitivities to Cu, Zn, Ni, and Co were consistently unaffected by BSO treatment, both in non-metallicolous and hypertolerant plants, suggesting that PC-based sequestration is not essential for constitutive tolerance or hypertolerance to these metals. Cd sensitivity was considerably increased by BSO, though exclusively in plants lacking Cd hypertolerance, suggesting that adaptive cadmium hypertolerance is not dependent on PC-mediated sequestration. BSO dramatically increased As sensitivity, both in non-adapted and As-hypertolerant plants, showing that PC-based sequestration is essential for both normal constitutive tolerance and adaptive hypertolerance to this metalloid. The primary function of PC synthase in plants and algae remains elusive.
Original languageEnglish
Pages (from-to)2381-2392
JournalJournal of Experimental Botany
Volume53
Issue number379
Publication statusPublished - 1 Dec 2002

Keywords

  • Buthionine sulphoximine
  • Heavy metal tolerance
  • Holcus lanatus
  • Hyperaccumulator
  • Metallophyte
  • Phytochelatins
  • Silene vulgaris
  • Thlaspi caerulescens

Fingerprint Dive into the research topics of 'The role of phytochelatins in constitutive and adaptive heavy metal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes'. Together they form a unique fingerprint.

  • Cite this

    Schat, H., Llugany, M., Vooijs, R., Hartley-Whitaker, J., & Bleeker, P. M. (2002). The role of phytochelatins in constitutive and adaptive heavy metal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes. Journal of Experimental Botany, 53(379), 2381-2392.