The role of 2', 3'-cyclic phosphodiesters in the bovine pancreatic ribonuclease A catalysed cleavage of RNA: intermediates or products?

Claudi M. Cuchillo, Xavier Parés, Alícia Guasch, Tom Barman, Franck Travers, M. Victòria Nogués

Research output: Contribution to journalArticleResearchpeer-review

63 Citations (Scopus)

Abstract

There is a considerable degree of ambiguity in the literature regarding the role of the 2',3'-cyclic phosphodiesters formed during the reaction of RNA cleavage catalysed by ribonuclease. Usually the reaction is considered to take place in two steps: in the first step there is a transphosphorylation of the RNA 3',5'-phosphodiester bond broken yielding a 2',3'-cyclic phosphodiester which in the second step is hydrolysed to a 3'-nucleotide. Although in many occasions, either explicitly or implicitly, the reaction is treated as taking place sequentially, this is not the case as it has been shown that the 2',3'-phosphodiesters are actually released to the medium as true products of the reaction and that no hydrolysis of these cyclic compounds takes place until all the susceptible 3',5'-phosphodiester bonds have been cyclised. Comparison of the hydrolysis and alcoholysis of the 2',3'-phosphodiesters catalysed by RNase A indicates that the hydrolysis reaction has to be considered formally as a special case of the transphosphorylation back reaction in which the R group of the R-OH substrate is just H. It is thus concluded that the 2',3'-cyclic phosphodiesters formed in the ribonuclease A reaction are true products of the transphosphorylation reaction and not intermediates as usually considered. © 1993.
Original languageEnglish
Pages (from-to)207-210
JournalFEBS Letters
Volume333
Issue number3
DOIs
Publication statusPublished - 1 Nov 1993

Keywords

  • 2',3'-Cyclic phosphodiester
  • Hydrolysis
  • Reaction intermediate
  • Ribonuclease A
  • Transphosphorylation

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