TY - JOUR
T1 - The restorative effect of intramuscular injection of tetanus toxin C-fragment in hemiparkinsonian rats
AU - Sánchez-González, Alejandra
AU - Mendieta, Liliana
AU - Palafox, Victoria
AU - Candalija, Anna
AU - Luna, Félix
AU - Aguilera, José
AU - Limón, Ilhuicamina Daniel
N1 - Funding Information:
This article was supported partially by VIEP-BUAP-2013 , given to Dr. Ilhuicamina Daniel Limón and the Ministerio de Ciencia e Innovación of the Spanish government , via the SAF2009-13626 grant given to Dr. José Aguilera. Alejandra Sánchez is a CONACyT scholarship holder ( 277954 ). All editing in English was carried out by Benjamin John Stewart.
Publisher Copyright:
© 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - The C-terminal domain of the heavy chain of tetanus toxin (Hc-TeTx) is a peptide that has a neuroprotective action against dopaminergic damage by MPP+, both in vitro and in vivo. The trophic effects of Hc-TeTx have been related to its ability to activate the pathways of the tropomyosin receptor kinase, which are crucial for survival process. Our group had previously shown neuroprotective effect of intramuscular Hc-TeTx treatment on animals with a dopaminergic lesion; however, there is no evidence indicating its restorative effects on advanced dopaminergic neurodegeneration. The aim of our study was to examine the restorative effects of an intramuscular injection of the Hc-TeTx fragment on the nigrostriatal system of hemiparkinsonian rats. The animals were administered with a vehicle or Hc-TeTx (20μg/kg) in the gastrocnemius muscle for three consecutive days post-dopaminergic lesion, which was made using 6-hydroxydopamine. Post-Hc-TeTx treatment, the hemiparkinsonian rats showed constant motor asymmetry. Moreover, the ipsilateral striatum of the post-Hc-TeTx group had a lower number of argyrophilic structures and a major immunorreactivity to Tyrosine Hydroxylase in the striatum and the substantia nigra pars compacta compared to the 6-OHDA group. Our results show the restorative effect of the Hc-TeTx fragment during the dopaminergic neurodegeneration caused by 6-OHDA.
AB - The C-terminal domain of the heavy chain of tetanus toxin (Hc-TeTx) is a peptide that has a neuroprotective action against dopaminergic damage by MPP+, both in vitro and in vivo. The trophic effects of Hc-TeTx have been related to its ability to activate the pathways of the tropomyosin receptor kinase, which are crucial for survival process. Our group had previously shown neuroprotective effect of intramuscular Hc-TeTx treatment on animals with a dopaminergic lesion; however, there is no evidence indicating its restorative effects on advanced dopaminergic neurodegeneration. The aim of our study was to examine the restorative effects of an intramuscular injection of the Hc-TeTx fragment on the nigrostriatal system of hemiparkinsonian rats. The animals were administered with a vehicle or Hc-TeTx (20μg/kg) in the gastrocnemius muscle for three consecutive days post-dopaminergic lesion, which was made using 6-hydroxydopamine. Post-Hc-TeTx treatment, the hemiparkinsonian rats showed constant motor asymmetry. Moreover, the ipsilateral striatum of the post-Hc-TeTx group had a lower number of argyrophilic structures and a major immunorreactivity to Tyrosine Hydroxylase in the striatum and the substantia nigra pars compacta compared to the 6-OHDA group. Our results show the restorative effect of the Hc-TeTx fragment during the dopaminergic neurodegeneration caused by 6-OHDA.
KW - C-terminal domain of the heavy chain of tetanus toxin
KW - Motor asymmetry
KW - Neurodegeneration
KW - Parkinson's disease
KW - Restoration
KW - Substantia nigra pars compacta
UR - http://www.scopus.com/inward/record.url?scp=84908398808&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.neures.2014.04.008
DO - https://doi.org/10.1016/j.neures.2014.04.008
M3 - Article
C2 - 24815514
VL - 84
SP - 1
EP - 9
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
ER -