The relationship between the chromophore moiety and the cation binding sites in bacteriorhodopsin

Mireia Duñach, Michel Seigneuret, Jean Louis Rigaud, Esteve Padrós

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleavage site probably located at the fifth loop, whereas in the blue membrane, the C-terminal tail is no longer susceptible to proteolysis. It is suggested that there exists a close relationship between the retinal environment and one or more of the cation binding sites. © 1986 Plenum Publishing Corporation.
Original languageEnglish
Pages (from-to)961-966
JournalBioscience Reports
Volume6
Issue number11
DOIs
Publication statusPublished - 1 Nov 1986

Keywords

  • bacteriorhodopsin
  • bleached membrane
  • cation binding sites
  • proteolysis
  • retinal

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