The proteome of human brain after ischemic stroke

Eloy Cuadrado, Anna Rosell, Nuria Colomé, Mar Hernández-Guillamon, Teresa García-Berrocoso, Marc Ribo, Alberto Alcazar, Arantxa Ortega-Aznar, Matilde Salinas, Francesc Canals, Joan Montaner

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29 Citations (Scopus)

Abstract

Although stroke is among the most common causes of death and chronic disability worldwide, the proteome of the ischemic human brain remains unknown. Only a few studies have investigated the ischemic brain proteome in rodent stroke models. We performed aproteomic study of the human brain after ischemic stroke usinga 2-dimensional differential gel electrophoresis-based proteomic approach. In brain samples from 6 deceased stroke patients and 3 control subjects, there was an average of 1,442 ± 231 protein spots in the gels. Changes of at least 1.5-fold in the relative expression of 132 protein spots between different cerebral areas (infarct core, peri-infarct, and contralateral tissue) were identified (p < 0.05); 39 of these were successfully identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Among the identified protein spots, we validated the results of 10 proteins by Western blot and determined the cellular localization in brain parenchyma for 3 of the identified proteins: dihydropyrimidinase-related protein 2, vesicle-fusing ATPase, and Rho dissociation inhibitor 1. These results contribute to understanding the processes that follow cerebral ischemia; moreover, some of the identified proteins may be therapeutic targets or biologic markers for determining the diagnosis and prognosis of stroke. © 2010 by the American Association of Neuropathologists, Inc.
Original languageEnglish
Pages (from-to)1105-1115
JournalJournal of Neuropathology and Experimental Neurology
Volume69
Issue number11
DOIs
Publication statusPublished - 1 Nov 2010

Keywords

  • Brain
  • DRP-2
  • Ischemia
  • N-ethylmaleimide sensitive factor
  • Proteomics
  • RhoGDI1
  • Stroke

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