TY - JOUR
T1 - The proteome of human brain after ischemic stroke
AU - Cuadrado, Eloy
AU - Rosell, Anna
AU - Colomé, Nuria
AU - Hernández-Guillamon, Mar
AU - García-Berrocoso, Teresa
AU - Ribo, Marc
AU - Alcazar, Alberto
AU - Ortega-Aznar, Arantxa
AU - Salinas, Matilde
AU - Canals, Francesc
AU - Montaner, Joan
PY - 2010/11/1
Y1 - 2010/11/1
N2 - Although stroke is among the most common causes of death and chronic disability worldwide, the proteome of the ischemic human brain remains unknown. Only a few studies have investigated the ischemic brain proteome in rodent stroke models. We performed aproteomic study of the human brain after ischemic stroke usinga 2-dimensional differential gel electrophoresis-based proteomic approach. In brain samples from 6 deceased stroke patients and 3 control subjects, there was an average of 1,442 ± 231 protein spots in the gels. Changes of at least 1.5-fold in the relative expression of 132 protein spots between different cerebral areas (infarct core, peri-infarct, and contralateral tissue) were identified (p < 0.05); 39 of these were successfully identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Among the identified protein spots, we validated the results of 10 proteins by Western blot and determined the cellular localization in brain parenchyma for 3 of the identified proteins: dihydropyrimidinase-related protein 2, vesicle-fusing ATPase, and Rho dissociation inhibitor 1. These results contribute to understanding the processes that follow cerebral ischemia; moreover, some of the identified proteins may be therapeutic targets or biologic markers for determining the diagnosis and prognosis of stroke. © 2010 by the American Association of Neuropathologists, Inc.
AB - Although stroke is among the most common causes of death and chronic disability worldwide, the proteome of the ischemic human brain remains unknown. Only a few studies have investigated the ischemic brain proteome in rodent stroke models. We performed aproteomic study of the human brain after ischemic stroke usinga 2-dimensional differential gel electrophoresis-based proteomic approach. In brain samples from 6 deceased stroke patients and 3 control subjects, there was an average of 1,442 ± 231 protein spots in the gels. Changes of at least 1.5-fold in the relative expression of 132 protein spots between different cerebral areas (infarct core, peri-infarct, and contralateral tissue) were identified (p < 0.05); 39 of these were successfully identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Among the identified protein spots, we validated the results of 10 proteins by Western blot and determined the cellular localization in brain parenchyma for 3 of the identified proteins: dihydropyrimidinase-related protein 2, vesicle-fusing ATPase, and Rho dissociation inhibitor 1. These results contribute to understanding the processes that follow cerebral ischemia; moreover, some of the identified proteins may be therapeutic targets or biologic markers for determining the diagnosis and prognosis of stroke. © 2010 by the American Association of Neuropathologists, Inc.
KW - Brain
KW - DRP-2
KW - Ischemia
KW - N-ethylmaleimide sensitive factor
KW - Proteomics
KW - RhoGDI1
KW - Stroke
U2 - 10.1097/NEN.0b013e3181f8c539
DO - 10.1097/NEN.0b013e3181f8c539
M3 - Article
SN - 0022-3069
VL - 69
SP - 1105
EP - 1115
JO - Journal of Neuropathology and Experimental Neurology
JF - Journal of Neuropathology and Experimental Neurology
IS - 11
ER -