The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii

Marc Gaona; Jordi Corral; Susana Campoy; Jordi Barbé; María Pérez−Varela; Jesús Aranda

doi:10.1128/aac.00712-24

The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii

Marc Gaona, Jordi Corral, Susana Campoy, Jordi Barbé, María Pérez−Varela, Jesús Aranda

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Acinetobacter baumannii is a notorious opportunistic pathogen responsible for healthcare-associated infections worldwide. Efflux pumps play crucial roles in mediating antimicrobial resistance, motility, and virulence. In this study, we present the identification and characterization of the new A. baumannii efflux pump SxtP belonging to the MFS superfamily (major facilitator superfamily), along with its associated activator LysR-type transcriptional regulator (LTTR) SxtR, demonstrating their roles in sulfamethoxazole/trimethoprim (also known as co-trimoxazole or SXT) resistance, surface-associated motility and virulence.

Original language	English
Article number	e00712-24
Number of pages	6
Journal	Antimicrobial Agents and Chemotherapy
Volume	68
Issue number	10
Early online date	28 Aug 2024
DOIs	https://doi.org/10.1128/aac.00712-24
Publication status	Published - 28 Aug 2024

Keywords

Acinetobacter
LysR-type transcriptional regulator
MFS efflux pump
co-trimoxazole
Acinetobacter baumannii/genetics
Drug Resistance, Multiple, Bacterial/genetics
Transcription Factors/genetics
Anti-Bacterial Agents/pharmacology
Virulence/genetics
Membrane Transport Proteins/genetics
Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology
Microbial Sensitivity Tests
Animals
Bacterial Proteins/genetics
Mice
Gene Expression Regulation, Bacterial
Acinetobacter Infections/microbiology

UN SDGs

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Gaona, M., Corral, J., Campoy, S., Barbé, J., Pérez−Varela, M., & Aranda, J. (2024). The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy, 68(10), Article e00712-24. https://doi.org/10.1128/aac.00712-24

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abstract = "Acinetobacter baumannii is a notorious opportunistic pathogen responsible for healthcare-associated infections worldwide. Efflux pumps play crucial roles in mediating antimicrobial resistance, motility, and virulence. In this study, we present the identification and characterization of the new A. baumannii efflux pump SxtP belonging to the MFS superfamily (major facilitator superfamily), along with its associated activator LysR-type transcriptional regulator (LTTR) SxtR, demonstrating their roles in sulfamethoxazole/trimethoprim (also known as co-trimoxazole or SXT) resistance, surface-associated motility and virulence.",

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author = "Marc Gaona and Jordi Corral and Susana Campoy and Jordi Barb{\'e} and Mar{\'i}a P{\'e}rez−Varela and Jes{\'u}s Aranda",

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year = "2024",

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day = "28",

doi = "10.1128/aac.00712-24",

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Gaona, M, Corral, J, Campoy, S, Barbé, J, Pérez−Varela, M & Aranda, J 2024, 'The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii', Antimicrobial Agents and Chemotherapy, vol. 68, no. 10, e00712-24. https://doi.org/10.1128/aac.00712-24

The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii. / Gaona, Marc; Corral, Jordi; Campoy, Susana et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 68, No. 10, e00712-24, 28.08.2024.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii

AU - Gaona, Marc

AU - Corral, Jordi

AU - Campoy, Susana

AU - Barbé, Jordi

AU - Pérez−Varela, María

AU - Aranda, Jesús

PY - 2024/8/28

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N2 - Acinetobacter baumannii is a notorious opportunistic pathogen responsible for healthcare-associated infections worldwide. Efflux pumps play crucial roles in mediating antimicrobial resistance, motility, and virulence. In this study, we present the identification and characterization of the new A. baumannii efflux pump SxtP belonging to the MFS superfamily (major facilitator superfamily), along with its associated activator LysR-type transcriptional regulator (LTTR) SxtR, demonstrating their roles in sulfamethoxazole/trimethoprim (also known as co-trimoxazole or SXT) resistance, surface-associated motility and virulence.

AB - Acinetobacter baumannii is a notorious opportunistic pathogen responsible for healthcare-associated infections worldwide. Efflux pumps play crucial roles in mediating antimicrobial resistance, motility, and virulence. In this study, we present the identification and characterization of the new A. baumannii efflux pump SxtP belonging to the MFS superfamily (major facilitator superfamily), along with its associated activator LysR-type transcriptional regulator (LTTR) SxtR, demonstrating their roles in sulfamethoxazole/trimethoprim (also known as co-trimoxazole or SXT) resistance, surface-associated motility and virulence.

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KW - LysR-type transcriptional regulator

KW - MFS efflux pump

KW - co-trimoxazole

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KW - Drug Resistance, Multiple, Bacterial/genetics

KW - Transcription Factors/genetics

KW - Anti-Bacterial Agents/pharmacology

KW - Virulence/genetics

KW - Membrane Transport Proteins/genetics

KW - Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology

KW - Microbial Sensitivity Tests

KW - Animals

KW - Bacterial Proteins/genetics

KW - Mice

KW - Gene Expression Regulation, Bacterial

KW - Acinetobacter Infections/microbiology

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DO - 10.1128/aac.00712-24

M3 - Article

C2 - 39194207

SN - 0066-4804

VL - 68

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

M1 - e00712-24

ER -

Gaona M, Corral J, Campoy S, Barbé J, Pérez−Varela M, Aranda J. The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy. 2024 Aug 28;68(10):e00712-24. Epub 2024 Aug 28. doi: 10.1128/aac.00712-24

The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii

Abstract

Keywords

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