The insertion element IS6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain

Ernesto Liébana, Alicia Aranaz, Lucas Dominguez, Ana Mateos, Oscar González-Llamazares, Elias F. Rodriguez-Ferri, Mariano Domingo, Dolores Vidal, Debby Cousins

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    28 Citations (Scopus)

    Abstract

    A total of 129 Mycobacterium bovis strains from 5 different Spanish locations were fingerprinted using the IS6110 repetitive element. We demonstrated the presence of multiple copies (from 2 to 13) of IS6110 in a large proportion (47.4%) of the M. bovis strains isolated from cattle and we showed that these strains can be successfully differentiated by means of the RFLP with IS6110. All of the M. bovis strains isolated from goats had multiple copies of IS6110 and 4 bands of 2, 1.7, 1.4 and 1.3 kb were common in all the caprine RFLP patterns. The caprine strains formed a clearly separate cluster from the bovine strains.
    Original languageEnglish
    Pages (from-to)223-233
    JournalVeterinary Microbiology
    Volume54
    Issue number3-4
    DOIs
    Publication statusPublished - 1 Mar 1997

    Keywords

    • cattle-bacteria
    • DNA fingerprinting
    • goat
    • IS6110
    • Mycobacterium bovis

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