The genotoxic effect of β-propiolactone on mammalian oocytes

J. Santaló; A. M. Estop; J. Egozcue

doi:10.1016/0165-1218(87)90050-4

The genotoxic effect of β-propiolactone on mammalian oocytes

J. Santaló, A. M. Estop, J. Egozcue

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

β-Propiolactone (βPL) has been tested on preimplantation mouse embryos for possible genotoxic effects. Tests were performed at different stages of meiosis (late prophase I, diakinesis/metaphase I, anaphase I, telophase I/prophase II and metaphase II) by injecting females at various times after the induction of superovulation. Male and female derived chromosome complements from first-cleavage embryos were analysed before syngamy for cytogenetic abnormalities. A higher proportion of diploid oocytes, produced by the non-extrusion of the first or second polar body, was found after fertilization when the compound was administered immediately before metaphase I or II. No obvious effect was detected at any other time of βPL exposure. Based on these results, several possible modes of action for βPL are postulated. © 1987.

Original language	English
Pages (from-to)	407-416
Journal	Mutation Research/Genetic Toxicology
Volume	189
Issue number	4
DOIs	https://doi.org/10.1016/0165-1218(87)90050-4
Publication status	Published - 1 Jan 1987

Keywords

(Mammalian oocytes)
(Mouse embryos)
Meiosis, different stages
β-Propiolactone

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10.1016/0165-1218(87)90050-4

Cite this

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title = "The genotoxic effect of β-propiolactone on mammalian oocytes",

abstract = "β-Propiolactone (βPL) has been tested on preimplantation mouse embryos for possible genotoxic effects. Tests were performed at different stages of meiosis (late prophase I, diakinesis/metaphase I, anaphase I, telophase I/prophase II and metaphase II) by injecting females at various times after the induction of superovulation. Male and female derived chromosome complements from first-cleavage embryos were analysed before syngamy for cytogenetic abnormalities. A higher proportion of diploid oocytes, produced by the non-extrusion of the first or second polar body, was found after fertilization when the compound was administered immediately before metaphase I or II. No obvious effect was detected at any other time of βPL exposure. Based on these results, several possible modes of action for βPL are postulated. {\textcopyright} 1987.",

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author = "J. Santal{\'o} and Estop, {A. M.} and J. Egozcue",

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doi = "10.1016/0165-1218(87)90050-4",

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TY - JOUR

T1 - The genotoxic effect of β-propiolactone on mammalian oocytes

AU - Santaló, J.

AU - Estop, A. M.

AU - Egozcue, J.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - β-Propiolactone (βPL) has been tested on preimplantation mouse embryos for possible genotoxic effects. Tests were performed at different stages of meiosis (late prophase I, diakinesis/metaphase I, anaphase I, telophase I/prophase II and metaphase II) by injecting females at various times after the induction of superovulation. Male and female derived chromosome complements from first-cleavage embryos were analysed before syngamy for cytogenetic abnormalities. A higher proportion of diploid oocytes, produced by the non-extrusion of the first or second polar body, was found after fertilization when the compound was administered immediately before metaphase I or II. No obvious effect was detected at any other time of βPL exposure. Based on these results, several possible modes of action for βPL are postulated. © 1987.

AB - β-Propiolactone (βPL) has been tested on preimplantation mouse embryos for possible genotoxic effects. Tests were performed at different stages of meiosis (late prophase I, diakinesis/metaphase I, anaphase I, telophase I/prophase II and metaphase II) by injecting females at various times after the induction of superovulation. Male and female derived chromosome complements from first-cleavage embryos were analysed before syngamy for cytogenetic abnormalities. A higher proportion of diploid oocytes, produced by the non-extrusion of the first or second polar body, was found after fertilization when the compound was administered immediately before metaphase I or II. No obvious effect was detected at any other time of βPL exposure. Based on these results, several possible modes of action for βPL are postulated. © 1987.

KW - (Mammalian oocytes)

KW - (Mouse embryos)

KW - Meiosis, different stages

KW - β-Propiolactone

U2 - 10.1016/0165-1218(87)90050-4

DO - 10.1016/0165-1218(87)90050-4

M3 - Article

SN - 0165-1218

VL - 189

SP - 407

EP - 416

JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

IS - 4

ER -

The genotoxic effect of β-propiolactone on mammalian oocytes

Abstract

Keywords

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