The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation

Josep Clotet, Francesc Posas, Antonio Casamayor, Ine Schaaff-Gerstenschläger, Joaquin Arinõ

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells. © 1991 Springer-Verlag.
Original languageEnglish
Pages (from-to)339-342
JournalCurrent Genetics
Volume19
DOIs
Publication statusPublished - 1 May 1991

Keywords

  • Gene disruption
  • Glycogen phosphorylase
  • Protein phosphatases

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