The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation

Josep Clotet; Francesc Posas; Antonio Casamayor; Ine Schaaff-Gerstenschläger; Joaquin Arinõ

doi:https://doi.org/10.1007/BF00309593

The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation

Josep Clotet, Francesc Posas, Antonio Casamayor, Ine Schaaff-Gerstenschläger, Joaquin Arinõ

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells. © 1991 Springer-Verlag.

Original language	English
Pages (from-to)	339-342
Journal	Current Genetics
Volume	19
DOIs	https://doi.org/10.1007/BF00309593
Publication status	Published - 1 May 1991

Keywords

Gene disruption
Glycogen phosphorylase
Protein phosphatases

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title = "The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation",

abstract = "S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted {"}in vitro{"}. Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells. {\textcopyright} 1991 Springer-Verlag.",

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year = "1991",

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T1 - The gene DIS2S1 is essential in Saccharomyces cerevisiae and is involved in glycogen phosphorylase activation

AU - Clotet, Josep

AU - Posas, Francesc

AU - Casamayor, Antonio

AU - Schaaff-Gerstenschläger, Ine

AU - Arinõ, Joaquin

PY - 1991/5/1

Y1 - 1991/5/1

N2 - S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells. © 1991 Springer-Verlag.

AB - S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells. © 1991 Springer-Verlag.

KW - Gene disruption

KW - Glycogen phosphorylase

KW - Protein phosphatases

U2 - https://doi.org/10.1007/BF00309593

DO - https://doi.org/10.1007/BF00309593

M3 - Article

VL - 19

SP - 339

EP - 342

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

ER -