Abstract
S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase a and glycogen synthase activity ratio were measured in diploids carrying a disrupted allele of the gene. Phosphorylase was dramatically activated in mutant cells but, under the same conditions, glycogen synthase activity was essentially identical in both mutant and wild-type cells. © 1991 Springer-Verlag.
Original language | English |
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Pages (from-to) | 339-342 |
Journal | Current Genetics |
Volume | 19 |
DOIs | |
Publication status | Published - 1 May 1991 |
Keywords
- Gene disruption
- Glycogen phosphorylase
- Protein phosphatases