The functional quality of soluble recombinant polypeptides produced in Escherichia coli is defined by a wide conformational spectrum

Research output: Contribution to journalArticleResearchpeer-review

26 Citations (Scopus)

Abstract

We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)7431-7433
JournalApplied and Environmental Microbiology
Volume74
Issue number23
DOIs
Publication statusPublished - 1 Dec 2008

Fingerprint Dive into the research topics of 'The functional quality of soluble recombinant polypeptides produced in Escherichia coli is defined by a wide conformational spectrum'. Together they form a unique fingerprint.

Cite this