We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
|Journal||Applied and Environmental Microbiology|
|Publication status||Published - 1 Dec 2008|