TY - JOUR
T1 - The functional quality of soluble recombinant polypeptides produced in Escherichia coli is defined by a wide conformational spectrum
AU - Martínez-Alonso, Mónica
AU - González-Montalbán, Nuria
AU - García-Fruitós, Elena
AU - Villaverde, Antonio
PY - 2008/12/1
Y1 - 2008/12/1
N2 - We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
AB - We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
U2 - 10.1128/AEM.01446-08
DO - 10.1128/AEM.01446-08
M3 - Article
VL - 74
SP - 7431
EP - 7433
IS - 23
ER -