TY - JOUR
T1 - The estrogen receptor fusion system in mouse models: a reversible switch
AU - Whitfield, Jonathan
AU - Littlewood, Trevor
AU - Evan, Gerard I.
AU - Soucek, Laura
PY - 2015/3/1
Y1 - 2015/3/1
N2 - © 2015 Cold Spring Harbor Laboratory Press. Reversible regulatory mouse models have significantly contributed to our understanding of normal tissue and cancer biology, providing the opportunity to temporally control initiation, progression, and evolution of physiological and pathological events. The tamoxifen inducible system, one of the best-characterized "reversible switch" models, has a number of beneficial features. In this system, the hormone-binding domain of the mammalian estrogen receptor is used as a heterologous regulatory domain. Upon ligand binding, the receptor is released from its inhibitory complex and the fusion protein becomes functional. We summarize the advantages and drawbacks of the system, describe several mouse models that rely on it, and discuss potential improvements that could render it even more useful and versatile.
AB - © 2015 Cold Spring Harbor Laboratory Press. Reversible regulatory mouse models have significantly contributed to our understanding of normal tissue and cancer biology, providing the opportunity to temporally control initiation, progression, and evolution of physiological and pathological events. The tamoxifen inducible system, one of the best-characterized "reversible switch" models, has a number of beneficial features. In this system, the hormone-binding domain of the mammalian estrogen receptor is used as a heterologous regulatory domain. Upon ligand binding, the receptor is released from its inhibitory complex and the fusion protein becomes functional. We summarize the advantages and drawbacks of the system, describe several mouse models that rely on it, and discuss potential improvements that could render it even more useful and versatile.
U2 - 10.1101/pdb.top069815
DO - 10.1101/pdb.top069815
M3 - Article
VL - 2015
SP - 227
EP - 234
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
IS - 3
ER -