The effect of TGF-β1 on cell proliferation and proteoglycan production in human melanoma cells depends on the degree of cell differentiation

Ascensión Heredia; Juan Villena; Manuel Romarís; Anna Molist; Anna Bassols

doi:10.1016/S0304-3835(96)04402-3

The effect of TGF-β1 on cell proliferation and proteoglycan production in human melanoma cells depends on the degree of cell differentiation

Ascensión Heredia, Juan Villena, Manuel Romarís, Anna Molist, Anna Bassols

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

The effect of transforming growth factor β1 (TGF-β1) on cell proliferation, colony formation in soft agar and synthesis and structure of proteoglycans was studied in three human melanoma cell lines at different stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3.44 (intermediate) and SK-mel-23 (late). TGF-β1 potently inhibited cell growth in monolayer as well as in soft agar. TGF-β1 increased the release of sulfated proteoglycans into the medium, including the cell-specific melanoma proteoglycan, mel-PG, and induced changes in disaccharide composition and sulfation of the glycosaminoglycan chains. In all the cases, the effect of TGF-β1 was more pronounced in the most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.44, whereas it had no effect on the most differentiated SK-mel-23 cells.

Original language	English
Pages (from-to)	39-47
Journal	Cancer Letters
Volume	109
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(96)04402-3
Publication status	Published - 3 Dec 1996

Keywords

Differentiation
Melanoma
Proteoglycan
Transforming growth factor β1

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title = "The effect of TGF-β1 on cell proliferation and proteoglycan production in human melanoma cells depends on the degree of cell differentiation",

abstract = "The effect of transforming growth factor β1 (TGF-β1) on cell proliferation, colony formation in soft agar and synthesis and structure of proteoglycans was studied in three human melanoma cell lines at different stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3.44 (intermediate) and SK-mel-23 (late). TGF-β1 potently inhibited cell growth in monolayer as well as in soft agar. TGF-β1 increased the release of sulfated proteoglycans into the medium, including the cell-specific melanoma proteoglycan, mel-PG, and induced changes in disaccharide composition and sulfation of the glycosaminoglycan chains. In all the cases, the effect of TGF-β1 was more pronounced in the most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.44, whereas it had no effect on the most differentiated SK-mel-23 cells.",

keywords = "Differentiation, Melanoma, Proteoglycan, Transforming growth factor β1",

author = "Ascensi{\'o}n Heredia and Juan Villena and Manuel Romar{\'i}s and Anna Molist and Anna Bassols",

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T1 - The effect of TGF-β1 on cell proliferation and proteoglycan production in human melanoma cells depends on the degree of cell differentiation

AU - Heredia, Ascensión

AU - Villena, Juan

AU - Romarís, Manuel

AU - Molist, Anna

AU - Bassols, Anna

PY - 1996/12/3

Y1 - 1996/12/3

N2 - The effect of transforming growth factor β1 (TGF-β1) on cell proliferation, colony formation in soft agar and synthesis and structure of proteoglycans was studied in three human melanoma cell lines at different stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3.44 (intermediate) and SK-mel-23 (late). TGF-β1 potently inhibited cell growth in monolayer as well as in soft agar. TGF-β1 increased the release of sulfated proteoglycans into the medium, including the cell-specific melanoma proteoglycan, mel-PG, and induced changes in disaccharide composition and sulfation of the glycosaminoglycan chains. In all the cases, the effect of TGF-β1 was more pronounced in the most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.44, whereas it had no effect on the most differentiated SK-mel-23 cells.

AB - The effect of transforming growth factor β1 (TGF-β1) on cell proliferation, colony formation in soft agar and synthesis and structure of proteoglycans was studied in three human melanoma cell lines at different stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3.44 (intermediate) and SK-mel-23 (late). TGF-β1 potently inhibited cell growth in monolayer as well as in soft agar. TGF-β1 increased the release of sulfated proteoglycans into the medium, including the cell-specific melanoma proteoglycan, mel-PG, and induced changes in disaccharide composition and sulfation of the glycosaminoglycan chains. In all the cases, the effect of TGF-β1 was more pronounced in the most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.44, whereas it had no effect on the most differentiated SK-mel-23 cells.

KW - Differentiation

KW - Melanoma

KW - Proteoglycan

KW - Transforming growth factor β1

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DO - 10.1016/S0304-3835(96)04402-3

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SP - 39

EP - 47

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

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