The effect of cytochalasin-B concentration on the frequency of micronuclei induced by four standard mutagens. Results from two laboratories

J. Surrallés, A. Antoccia, A. Creus, F. Degrassi, F. Peris, C. Tanzarella, N. Xamena, R. Marcos

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Abstract

In a previous collaborative work, we have recently shown that the cytochalasin-B (Cyt-B) concentration used in the human lymphocytes cytokinesis-block micronucleus (CBMN) assay is an important variable in the baseline micronuclei (MN) frequency as well as in the percentage of binucleated cells obtained. Now we have investigated how Cyt-B concentration modulates the MN frequency induced in whole blood human lymphocyte cultures by two clastogens (ethyl methanesulphonate and mitomycin-C) and two aneugens (colchicine and vincristine sulphate). The experimental design includes six donors, two concentrations of Cyt-B (3 and 6 g/ml), two concentrations of the four chemicals tested and the exchange of slides between laboratories. The statistical analysls of the results shows: (i) non-significant differences in the MN frequencies and in the toxicity results between scorers from each laboratory, except for 0.06 M colchicine at 3 g/ml Cyt-B; (ii) an induction of MN by all genotoxic agents tested, the frequencies being lower with 6 than with 3 g/ml Cyt-B, in control and aneugen-treated cultures; and (iii) significant differences between Cyt-B concentrations in several treatments, obtaining lower MN frequencies and higher values for nuclear division index and % binucleated cells when 6 g/ml Cyt-B was used. Bearing in mind these results as well as the toxicity data showing that 6 g/ml Cyt-B is much more effective in blocking cytokinesis, we can conclude that the use of 3g/ml Cyt-B may overestimate the induced frequency of MN. © 1994 Oxford University Press.
Original languageEnglish
Pages (from-to)347-353
JournalMutagenesis
Volume9
DOIs
Publication statusPublished - 1 Jan 1994

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