The CaM-kinase II inhibitor KN-62 blocks NMDA but not kainate stimulation of NO synthesis

José Rodriguez-Alvarez, Mireille Lafon-Cazal, Joël Bockaert

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9 Citations (Scopus)

Abstract

We have studied the effect of CaM-kinase II inhibition induced by KN-62 on NO synthesis mediated by the activation of NMDA and kainate receptors in striatal neurones. KN-62 partially inhibited NMDA-mediated NO synthesis but the effect of kainate on NO production was unaffected by the specific CaM-kinase II inhibitor. Ionomycin-induced cGMP production and the NMDA-mediated superoxide generation by nNOS were also inhibited by KN-62. Since we determined the NO synthesis via its ability to increase intracellular cGMP, we checked that the kinase inhibitor was unable to block the cGMP production induced by a NO-donor like 3-morpholinosydnonimine (SIN-1). In conclusion, these results indicate that, depending on the glutamatergic receptors involved, CaM-kinase II could participate in the regulation of NO synthesis in striatal neurones.
Original languageEnglish
Pages (from-to)2525-2528
JournalNeuroReport
Volume7
Issue number15-17
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • CaM-kinase II
  • Glutamate receptors
  • Nitric oxide
  • Striatal neurones

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