Technical improvements in corrosion casting of small specimens: A study on mesonephric tubules and vessels of chicken embryos

A. Carretero; H. Ditrich; M. Navarro; H. Splechtna; J. Ruberte; G. J. Burton; S. Yoshida

Technical improvements in corrosion casting of small specimens: A study on mesonephric tubules and vessels of chicken embryos

A. Carretero, H. Ditrich, M. Navarro, H. Splechtna, J. Ruberte, G. J. Burton, S. Yoshida

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The injection technique for corrosion casting of small, embryonic material can be improved by using a 'chemical ligature' (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.

Original language	English
Pages (from-to)	1333-1338
Journal	Scanning Microscopy
Volume	7
Issue number	4
Publication status	Published - 1 Dec 1993

Keywords

blood vessels
chicken embryo
Corrosion casts
development
embryology
injection technique
kidney
renal tubules

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title = "Technical improvements in corrosion casting of small specimens: A study on mesonephric tubules and vessels of chicken embryos",

abstract = "The injection technique for corrosion casting of small, embryonic material can be improved by using a 'chemical ligature' (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.",

keywords = "blood vessels, chicken embryo, Corrosion casts, development, embryology, injection technique, kidney, renal tubules",

author = "A. Carretero and H. Ditrich and M. Navarro and H. Splechtna and J. Ruberte and Burton, {G. J.} and S. Yoshida",

year = "1993",

month = dec,

day = "1",

language = "English",

volume = "7",

pages = "1333--1338",

number = "4",

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Technical improvements in corrosion casting of small specimens: A study on mesonephric tubules and vessels of chicken embryos. / Carretero, A.; Ditrich, H.; Navarro, M.; Splechtna, H.; Ruberte, J.; Burton, G. J.; Yoshida, S.

In: Scanning Microscopy, Vol. 7, No. 4, 01.12.1993, p. 1333-1338.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Technical improvements in corrosion casting of small specimens: A study on mesonephric tubules and vessels of chicken embryos

AU - Carretero, A.

AU - Ditrich, H.

AU - Navarro, M.

AU - Splechtna, H.

AU - Ruberte, J.

AU - Burton, G. J.

AU - Yoshida, S.

PY - 1993/12/1

Y1 - 1993/12/1

N2 - The injection technique for corrosion casting of small, embryonic material can be improved by using a 'chemical ligature' (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.

AB - The injection technique for corrosion casting of small, embryonic material can be improved by using a 'chemical ligature' (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.

KW - blood vessels

KW - chicken embryo

KW - Corrosion casts

KW - development

KW - embryology

KW - injection technique

KW - kidney

KW - renal tubules

M3 - Article

VL - 7

SP - 1333

EP - 1338

IS - 4

ER -