Technical improvements in corrosion casting of small specimens: A study on mesonephric tubules and vessels of chicken embryos

A. Carretero, H. Ditrich, M. Navarro, H. Splechtna, J. Ruberte, G. J. Burton, S. Yoshida

    Research output: Contribution to journalArticleResearchpeer-review

    17 Citations (Scopus)

    Abstract

    The injection technique for corrosion casting of small, embryonic material can be improved by using a 'chemical ligature' (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.
    Original languageEnglish
    Pages (from-to)1333-1338
    JournalScanning Microscopy
    Volume7
    Issue number4
    Publication statusPublished - 1 Dec 1993

    Keywords

    • blood vessels
    • chicken embryo
    • Corrosion casts
    • development
    • embryology
    • injection technique
    • kidney
    • renal tubules

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