Abstract
The injection technique for corrosion casting of small, embryonic material can be improved by using a 'chemical ligature' (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.
Original language | English |
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Pages (from-to) | 1333-1338 |
Journal | Scanning Microscopy |
Volume | 7 |
Issue number | 4 |
Publication status | Published - 1 Dec 1993 |
Keywords
- blood vessels
- chicken embryo
- Corrosion casts
- development
- embryology
- injection technique
- kidney
- renal tubules