Taxonomic identification of different species of the genus aeromonas by whole-genome sequencing and use of their species-specific Β-lactamases as phylogenetic markers

Xavier Bertran, Marc Rubio, Laura Gómez, Teresa Llovet, Carme Muñoz, Ferran Navarro*, Elisenda Miro

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Some Aeromonas species, potentially pathogenic for humans, are known to express up to three different classes of chromosomal Β-lactamases, which may become hyperproduced and cause treatment failure. The aim of this study was to assess the utility of these species-specific Β-lactamase genes as phylogenetic markers using whole-genome sequencing data. Core-genome alignments were generated for 36 Aeromonas genomes from seven different species and scanned for antimicrobial resistance genes. Core-genome alignment confirmed the MALDI-TOF identification of most of the isolates and re-identified an A. hydrophila isolate as A. dhakensis. Three (B, C and D) of the four Ambler classes of Β-lactamase genes were found in A. sobria, A. allosacharophila, A. hydrophila and A. dhakensis (blaCphA, blaAmpC and blaOXA). A. veronii only showed class-B- and class-D-like matches (blaCphA and blaOXA), whereas those for A. media, A. rivipollensis and A. caviae were class C and D (blaCMY, blaMOX and blaOXA427). The phylogenetic tree derived from concatenated sequences of Β-lactamase genes successfully clustered each species. Some isolates also had resistance to sulfonamides, quinolones and aminoglycosides. Whole-genome sequencing proved to be a useful method to identify Aeromonas at the species level, which led to the unexpected identification of A. dhakensis and A.rivipollensis and revealed the resistome of each isolate.

Original languageEnglish
Article number354
JournalAntibiotics
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Aeromonas dhakensis
  • Aeromonas rivipollensis
  • Beta-lactamases
  • Cephamicinases
  • Core-genome
  • FOX
  • MOX

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