Systemic administration of 3-nitropropionic acid points out a different role for active caspase-3 in neurons and astrocytes

Joaquim Duran-Vilaregut, Jaume del Valle, Gemma Manich, Fèlix Junyent, Antoni Camins, Mercè Pallàs, Carme Pelegrí, Jordi Vilaplana*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

The intraperitoneal administration of 3-nitropropionic acid, which is commonly used to induce toxicity models of Huntington's disease in experimental animals, produces severe brain injury in the lateral part of the striatum. We studied the presence of active caspase-3 in neurons and astrocytes from brains of rats treated with 3-nitropropionic acid following a subacute administration protocol. Active caspase-3 was almost absent in the core of the striatal lesion. However, it was expressed, albeit weakly, in the neurons present in the rim of the lesion. In cortex and non-injured striatal areas, and in the cortex and striatum of control animals, active caspase-3 staining was widely distributed and vivid, but localized in the cell bodies of astrocytes rather than in neurons. In treated animals, some of the active caspase-3 positive neurons localized in the rim of the lesion were also positive for TUNEL staining. This indicates the presence of a caspase-mediated apoptotic process. TUNEL was not present in control animals or in the astrocytes of treated animals. Thus, the presence of active caspase-3 in astrocytes may be merely constitutive.

Original languageAmerican English
Pages (from-to)443-450
Number of pages8
JournalNeurochemistry International
Volume56
Issue number3
DOIs
Publication statusPublished - Feb 2010

Keywords

  • Apoptosis
  • Huntington's disease
  • Neurodegeneration
  • Rat
  • Striatum
  • TUNEL

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