Synthesis of sulfated bioactive peptides using immobilized arylsulfotransferase from Eubacterium sp

Bernard Delhom; Gregorio Alvaro; Gloria Caminal; Josep Lluís Torres; Pere Clapés

doi:10.1007/BF00140212

Synthesis of sulfated bioactive peptides using immobilized arylsulfotransferase from Eubacterium sp

Bernard Delhom, Gregorio Alvaro, Gloria Caminal, Josep Lluís Torres, Pere Clapés

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Abstract

An arylsulfotransferase from Eubacterium sp. was immobilized on agarose gels by multipoint covalent attachment. The yield of immobilization was 80% with an activity of 11 UA/ml of derivative. After 19 days of incubation, the loss of activity of the derivative was 36%. The immobilized preparation was used to transfer selectively a sulfate group from p-nitrophenolsulfate to selected tyrosine containing biologically active peptides in 92-99% of yield.

Original language	English
Pages (from-to)	609-614
Journal	Biotechnology Letters
Volume	18
Issue number	5
DOIs	https://doi.org/10.1007/BF00140212
Publication status	Published - 1 Jan 1996

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@article{4da95fef1f314db58c22a2121ec674de,

title = "Synthesis of sulfated bioactive peptides using immobilized arylsulfotransferase from Eubacterium sp",

abstract = "An arylsulfotransferase from Eubacterium sp. was immobilized on agarose gels by multipoint covalent attachment. The yield of immobilization was 80% with an activity of 11 UA/ml of derivative. After 19 days of incubation, the loss of activity of the derivative was 36%. The immobilized preparation was used to transfer selectively a sulfate group from p-nitrophenolsulfate to selected tyrosine containing biologically active peptides in 92-99% of yield.",

author = "Bernard Delhom and Gregorio Alvaro and Gloria Caminal and Torres, {Josep Llu{\'i}s} and Pere Clap{\'e}s",

year = "1996",

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doi = "10.1007/BF00140212",

language = "English",

volume = "18",

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T1 - Synthesis of sulfated bioactive peptides using immobilized arylsulfotransferase from Eubacterium sp

AU - Delhom, Bernard

AU - Alvaro, Gregorio

AU - Caminal, Gloria

AU - Torres, Josep Lluís

AU - Clapés, Pere

PY - 1996/1/1

Y1 - 1996/1/1

N2 - An arylsulfotransferase from Eubacterium sp. was immobilized on agarose gels by multipoint covalent attachment. The yield of immobilization was 80% with an activity of 11 UA/ml of derivative. After 19 days of incubation, the loss of activity of the derivative was 36%. The immobilized preparation was used to transfer selectively a sulfate group from p-nitrophenolsulfate to selected tyrosine containing biologically active peptides in 92-99% of yield.

AB - An arylsulfotransferase from Eubacterium sp. was immobilized on agarose gels by multipoint covalent attachment. The yield of immobilization was 80% with an activity of 11 UA/ml of derivative. After 19 days of incubation, the loss of activity of the derivative was 36%. The immobilized preparation was used to transfer selectively a sulfate group from p-nitrophenolsulfate to selected tyrosine containing biologically active peptides in 92-99% of yield.

U2 - 10.1007/BF00140212

DO - 10.1007/BF00140212

M3 - Article

VL - 18

SP - 609

EP - 614

IS - 5

ER -

Synthesis of sulfated bioactive peptides using immobilized arylsulfotransferase from Eubacterium sp

Abstract

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