TY - JOUR
T1 - Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells
AU - Oliveira, Elisabete
AU - Santos, Hugo M.
AU - Garcia-Pardo, Javier
AU - Diniz, Mário
AU - Lorenzo, Julia
AU - Rodríguez-González, Benito
AU - Capelo, José L.
AU - Lodeiro, Carlos
PY - 2013/12/5
Y1 - 2013/12/5
N2 - © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand.
AB - © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand.
KW - Cytotoxicity
KW - Fluorescence
KW - Goldfish
KW - Human hepatoma cells
KW - Silver nanoparticles
U2 - https://doi.org/10.3389/fchem.2013.00029
DO - https://doi.org/10.3389/fchem.2013.00029
M3 - Article
VL - 1
JO - Frontiers in Chemistry
JF - Frontiers in Chemistry
SN - 2296-2646
M1 - 29
ER -