Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells

Elisabete Oliveira; Hugo M. Santos; Javier Garcia-Pardo; Mário Diniz; Julia Lorenzo; Benito Rodríguez-González; José L. Capelo; Carlos Lodeiro

doi:https://doi.org/10.3389/fchem.2013.00029

Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells

Elisabete Oliveira, Hugo M. Santos, Javier Garcia-Pardo, Mário Diniz, Julia Lorenzo, Benito Rodríguez-González, José L. Capelo, Carlos Lodeiro

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

© 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand.

Original language	English
Article number	29
Journal	Frontiers in Chemistry
Volume	1
DOIs	https://doi.org/10.3389/fchem.2013.00029
Publication status	Published - 5 Dec 2013

Keywords

Cytotoxicity
Fluorescence
Goldfish
Human hepatoma cells
Silver nanoparticles

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Oliveira, E., Santos, H. M., Garcia-Pardo, J., Diniz, M., Lorenzo, J., Rodríguez-González, B., Capelo, J. L., & Lodeiro, C. (2013). Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells. Frontiers in Chemistry, 1, [29]. https://doi.org/10.3389/fchem.2013.00029

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title = "Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells",

abstract = "{\textcopyright} 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodr{\'i}guez-Gonz{\'a}lez, Capelo and Lodeiro. Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand.",

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Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells. / Oliveira, Elisabete; Santos, Hugo M.; Garcia-Pardo, Javier; Diniz, Mário; Lorenzo, Julia; Rodríguez-González, Benito; Capelo, José L.; Lodeiro, Carlos.

In: Frontiers in Chemistry, Vol. 1, 29, 05.12.2013.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Synthesis of functionalized fluorescent silver nanoparticles and their toxicological effect in aquatic environments (Goldfish) and HEPG2 cells

AU - Oliveira, Elisabete

AU - Santos, Hugo M.

AU - Garcia-Pardo, Javier

AU - Diniz, Mário

AU - Lorenzo, Julia

AU - Rodríguez-González, Benito

AU - Capelo, José L.

AU - Lodeiro, Carlos

PY - 2013/12/5

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N2 - © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand.

AB - © 2013 Oliveira, Santos, Garcia-Pardo, Diniz, Lorenzo, Rodríguez-González, Capelo and Lodeiro. Silver nanoparticles, AgNPs, are widely used in our daily life, mostly due to their antibacterial, antiviral, and antifungal properties. However, their potential toxicity remains unclear. In order to unravel this issue, emissive AgNPs were first synthetized using an inexpensive photochemical method, and then their permeation was assessed in vivo in goldfish and in vitro in human hepatoma cells (HepG2). In addition, the oxidative stress caused by AgNPs was assessed in enzymes such as glutathione-S-transferase (GST), catalase (CAT), and in lipid peroxidation (LPO). This study demonstrates that the smallest sized AgNPs@3 promote the largest changes in gold fish livers, whereas AgNPs@1 were found to be toxic in HEPG2 cells depending on both the size and functionalized/stabilizer ligand.

KW - Cytotoxicity

KW - Fluorescence

KW - Goldfish

KW - Human hepatoma cells

KW - Silver nanoparticles

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