Synthesis of biodiesel from high FFA alperujo oil catalysed by immobilised lipase

Kírian Bonet-Ragel; Albert Canet; M. Dolors Benaiges; Francisco Valero

doi:https://doi.org/10.1016/j.fuel.2015.08.032

Synthesis of biodiesel from high FFA alperujo oil catalysed by immobilised lipase

Kírian Bonet-Ragel, Albert Canet, M. Dolors Benaiges, Francisco Valero

Research output: Contribution to journal › Article › Research › peer-review

33 Citations (Scopus)

Abstract

© 2015 Elsevier Ltd. All rights reserved. Synthesis of biodiesel was carried out through enzymatic biocatalysis by the transesterification of natural waste olive oil (alperujo oil) using a recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL). The most relevant property of this uncommon substrate is the high content of free fatty acids (FFAs), making it impossible to produce biodiesel through basic catalysis. The synthesis reactions were carried out in a solvent-free media using a covalent immobilisation on a glutaraldehyde-treated polymethacrylate amino-epoxide carrier. Methanol was added by stepwise addition to avoid lipase inactivation. FFAs effects on the kinetics, yields and stability of biocatalysts were studied. It was concluded that the high free fatty acids content allows an increasing on the initial reaction rate and it gives more stability to the immobilised enzyme, demonstrating the efficiency of this new substrate for the biodiesel production.

Original language	English
Pages (from-to)	12-17
Journal	Fuel
Volume	161
DOIs	https://doi.org/10.1016/j.fuel.2015.08.032
Publication status	Published - 24 Aug 2015

Keywords

Alperujo oil
Biodiesel synthesis
Free fatty acids effect
Rhizopus oryzae lipase

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title = "Synthesis of biodiesel from high FFA alperujo oil catalysed by immobilised lipase",

abstract = "{\textcopyright} 2015 Elsevier Ltd. All rights reserved. Synthesis of biodiesel was carried out through enzymatic biocatalysis by the transesterification of natural waste olive oil (alperujo oil) using a recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL). The most relevant property of this uncommon substrate is the high content of free fatty acids (FFAs), making it impossible to produce biodiesel through basic catalysis. The synthesis reactions were carried out in a solvent-free media using a covalent immobilisation on a glutaraldehyde-treated polymethacrylate amino-epoxide carrier. Methanol was added by stepwise addition to avoid lipase inactivation. FFAs effects on the kinetics, yields and stability of biocatalysts were studied. It was concluded that the high free fatty acids content allows an increasing on the initial reaction rate and it gives more stability to the immobilised enzyme, demonstrating the efficiency of this new substrate for the biodiesel production.",

keywords = "Alperujo oil, Biodiesel synthesis, Free fatty acids effect, Rhizopus oryzae lipase",

author = "K{\'i}rian Bonet-Ragel and Albert Canet and Benaiges, {M. Dolors} and Francisco Valero",

year = "2015",

month = aug,

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doi = "https://doi.org/10.1016/j.fuel.2015.08.032",

language = "English",

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pages = "12--17",

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TY - JOUR

T1 - Synthesis of biodiesel from high FFA alperujo oil catalysed by immobilised lipase

AU - Bonet-Ragel, Kírian

AU - Canet, Albert

AU - Benaiges, M. Dolors

AU - Valero, Francisco

PY - 2015/8/24

Y1 - 2015/8/24

N2 - © 2015 Elsevier Ltd. All rights reserved. Synthesis of biodiesel was carried out through enzymatic biocatalysis by the transesterification of natural waste olive oil (alperujo oil) using a recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL). The most relevant property of this uncommon substrate is the high content of free fatty acids (FFAs), making it impossible to produce biodiesel through basic catalysis. The synthesis reactions were carried out in a solvent-free media using a covalent immobilisation on a glutaraldehyde-treated polymethacrylate amino-epoxide carrier. Methanol was added by stepwise addition to avoid lipase inactivation. FFAs effects on the kinetics, yields and stability of biocatalysts were studied. It was concluded that the high free fatty acids content allows an increasing on the initial reaction rate and it gives more stability to the immobilised enzyme, demonstrating the efficiency of this new substrate for the biodiesel production.

AB - © 2015 Elsevier Ltd. All rights reserved. Synthesis of biodiesel was carried out through enzymatic biocatalysis by the transesterification of natural waste olive oil (alperujo oil) using a recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL). The most relevant property of this uncommon substrate is the high content of free fatty acids (FFAs), making it impossible to produce biodiesel through basic catalysis. The synthesis reactions were carried out in a solvent-free media using a covalent immobilisation on a glutaraldehyde-treated polymethacrylate amino-epoxide carrier. Methanol was added by stepwise addition to avoid lipase inactivation. FFAs effects on the kinetics, yields and stability of biocatalysts were studied. It was concluded that the high free fatty acids content allows an increasing on the initial reaction rate and it gives more stability to the immobilised enzyme, demonstrating the efficiency of this new substrate for the biodiesel production.

KW - Alperujo oil

KW - Biodiesel synthesis

KW - Free fatty acids effect

KW - Rhizopus oryzae lipase

U2 - https://doi.org/10.1016/j.fuel.2015.08.032

DO - https://doi.org/10.1016/j.fuel.2015.08.032

M3 - Article

VL - 161

SP - 12

EP - 17

JO - Fuel

JF - Fuel

SN - 0016-2361

ER -