Abstract
The synthesis and chemical characterization of two new trans platinum complexes, trans-[PtCl2NH3(4-hydroxymethylpyridine)] (1) and trans-[PtCl4NH3(4-hydroxymethylpyridine)] (2) are described. Their ability to interact with 5′-GMP by themselves and in the presence of reducing agents in the case of trans-[PtCl4NH 3(4-hydroxymethylpyridine)] were tested. Circular dichroism, electrophoretic mobility in agarose gel, and atomic force microscopy studies showed that the interaction of complex 1 with DNA is stronger than that of complex 2. Cytotoxicity tests against HL-60 tumor cells also showed higher activity for trans-[PtCl2NH3(4-hydroxymethylpyridine)] than for trans-[PtCl4NH3(4-hydroxymethylpyridine)]. Complex 1 presents similar behavior to cisplatin, but with a lower IC 50 at 24 h. Complex 1 also showed high apoptosis induction. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
Original language | English |
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Pages (from-to) | 2068-2077 |
Journal | ChemBioChem |
Volume | 6 |
DOIs | |
Publication status | Published - 1 Nov 2005 |
Keywords
- Ab initio calculations
- Antitumor agents
- Apoptosis
- Bioinorganic chemistry
- DNA
- Platinum