Sweat Testing for the Detection of Methylone after Controlled Administrations in Humans

Alessandro Di Giorgi, Giorgia Sprega, Lourdes Poyatos, Esther Papaseit, Clara Pérez-Mañá, Annagiulia Di Trana, Maria Rosaria Varì*, Francesco Paolo Busardò, Simona Pichini, Simona Zaami, Alfredo Fabrizio Lo Faro, Magí Farré

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review


The aim of this study was to determine the excretion of methylone and its metabolites in sweat following the ingestion of increasing controlled doses of 50, 100, 150 and 200 mg of methylone to twelve healthy volunteers involved in a clinical trial. Methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone (MDC) were analyzed in sweat patches by liquid chromatography–tandem mass spectrometry. Methylone and MDC were detected in sweat at 2 h and reached their highest accumulation (Cmax) at 24 h after the administration of 50, 100, 150 and 200 mg doses. In contrast, HMMC was not detectable at any time interval after each dose. Sweat proved to be a suitable matrix for methylone and its metabolites’ determination in clinical and toxicological studies, providing a concentration that reveals recent drug consumption.

Original languageEnglish
Article number7395
JournalInternational journal of molecular sciences
Issue number8
Publication statusPublished - Apr 2023


  • LC-MS/MS
  • methylone
  • pharmacokinetics
  • sweat


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