Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies

Witold I. Tatkiewicz; Joaquin Seras-Franzoso; Elena Garcia-Fruitós; Esther Vazquez; A. R. Kyvik; Judith Guasch; Antonio Villaverde; Jaume Veciana; Imma Ratera

doi:https://doi.org/10.1021/acsami.8b06821

Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies

Witold I. Tatkiewicz, Joaquin Seras-Franzoso, Elena Garcia-Fruitós, Esther Vazquez, A. R. Kyvik, Judith Guasch, Antonio Villaverde, Jaume Veciana, Imma Ratera

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Copyright © 2018 American Chemical Society. A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces "a-la-carte" with pNPs enabling different categories of high-throughput studies on cell motility.

Original language	English
Pages (from-to)	25779-25786
Journal	ACS Applied Materials & Interfaces
Volume	10
Issue number	30
DOIs	https://doi.org/10.1021/acsami.8b06821
Publication status	Published - 1 Aug 2018

Keywords

cell motility
coffee-drop effect
inclusion bodies
protein nanoparticles
surface biofunctionalization
surface gradient deposition

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https://doi.org/10.1021/acsami.8b06821

https://ddd.uab.cat/record/236683

Cite this

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title = "Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies",

abstract = "Copyright {\textcopyright} 2018 American Chemical Society. A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces {"}a-la-carte{"} with pNPs enabling different categories of high-throughput studies on cell motility.",

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T1 - Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies

AU - Tatkiewicz, Witold I.

AU - Seras-Franzoso, Joaquin

AU - Garcia-Fruitós, Elena

AU - Vazquez, Esther

AU - Kyvik, A. R.

AU - Guasch, Judith

AU - Villaverde, Antonio

AU - Veciana, Jaume

AU - Ratera, Imma

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Copyright © 2018 American Chemical Society. A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces "a-la-carte" with pNPs enabling different categories of high-throughput studies on cell motility.

AB - Copyright © 2018 American Chemical Society. A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces "a-la-carte" with pNPs enabling different categories of high-throughput studies on cell motility.

KW - cell motility

KW - coffee-drop effect

KW - inclusion bodies

KW - protein nanoparticles

KW - surface biofunctionalization

KW - surface gradient deposition

U2 - https://doi.org/10.1021/acsami.8b06821

DO - https://doi.org/10.1021/acsami.8b06821

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EP - 25786

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

IS - 30

ER -

Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies

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Keywords

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