Surface-Bound Gradient Deposition of Protein Nanoparticles for Cell Motility Studies

Witold I. Tatkiewicz, Joaquin Seras-Franzoso, Elena Garcia-Fruitós, Esther Vazquez, A. R. Kyvik, Judith Guasch, Antonio Villaverde, Jaume Veciana, Imma Ratera

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)


Copyright © 2018 American Chemical Society. A versatile evaporation-assisted methodology based on the coffee-drop effect is described to deposit nanoparticles on surfaces, obtaining for the first time patterned gradients of protein nanoparticles (pNPs) by using a simple custom-made device. Fully controllable patterns with specific periodicities consisting of stripes with different widths and distinct nanoparticle concentration as well as gradients can be produced over large areas (∼10 cm2) in a fast (up to 10 mm2/min), reproducible, and cost-effective manner using an operational protocol optimized by an evolutionary algorithm. The developed method opens the possibility to decorate surfaces "a-la-carte" with pNPs enabling different categories of high-throughput studies on cell motility.
Original languageEnglish
Pages (from-to)25779-25786
JournalACS Applied Materials & Interfaces
Issue number30
Publication statusPublished - 1 Aug 2018


  • cell motility
  • coffee-drop effect
  • inclusion bodies
  • protein nanoparticles
  • surface biofunctionalization
  • surface gradient deposition


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